概述

  • 产品名称Anti-PAK4抗体
    参阅全部 PAK4 一抗
  • 描述
    兔多克隆抗体to PAK4
  • 特异性This antibody reacts with PAK4
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A 13 amino acid synthetic peptide from near the center of human PAK4

  • 阳性对照
    • SW480 cell lysate and human colon tissue

性能

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应用

Our Abpromise guarantee covers the use of ab62509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
  • 组织特异性Highest expression in prostate, testis and colon.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated on serine residues when activated by CDC42/p21.
    Phosphorylated on tyrosine residues upon stimulation of FGFR2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1142 antibody
    • p21 activated kinase 4 antibody
    • p21 Cdc42/Rac1-actiated kinase 4 antibody
    • P21 protein (Cdc42/Rac) activated kinase 4 antibody
    • p21(CDKN1A) activated kinase 4 antibody
    • p21-activated kinase 4 antibody
    • PAK 4 antibody
    • PAK-4 antibody
    • Pak4 antibody
    • PAK4_HUMAN antibody
    • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody
    • Serine threonine kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK4 antibody
    • Serine/threonine-protein kinase PAK 4 antibody
    see all

Anti-PAK4 antibody 图像

  • Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/ml
    Lane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/ml

    Lane 1 : SW480 cell lysate
    Lane 2 : SW480 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • Immunohistochemistry of PAK4 in paraffin embedded human colon tissue section with ab62509 antibody at 10 µg/ml.
  • ICC/IF image of ab62509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of PAK4 in Human Colon cells using ab62509 at 20 ug/ml.

Anti-PAK4 antibody (ab62509)参考文献

This product has been referenced in:
  • Wen X  et al. ROCK1 and LIM Kinase Modulate Retrovirus Particle Release and Cell-Cell Transmission Events. J Virol 88:6906-21 (2014). Read more (PubMed: 24696479) »
  • Wang Z  et al. MiR-145 regulates PAK4 via the MAPK pathway and exhibits an antitumor effect in human colon cells. Biochem Biophys Res Commun : (2012). WB ; Human . Read more (PubMed: 22766504) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (SDS-PAGE 10%)
Sample Chinese Hamster Cell lysate - whole cell (CHO cells)
Specification CHO cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Sep 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"