概述

  • 产品名称
  • 描述
    兔多克隆抗体to PAK4
  • 宿主
    Rabbit
  • 特异性
    This antibody reacts with PAK4
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A 13 amino acid synthetic peptide from near the center of human PAK4

  • 阳性对照
    • SW480 cell lysate and human colon tissue

性能

应用

Our Abpromise guarantee covers the use of ab62509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能
    Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
  • 组织特异性
    Highest expression in prostate, testis and colon.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated on serine residues when activated by CDC42/p21.
    Phosphorylated on tyrosine residues upon stimulation of FGFR2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1142 antibody
    • p21 activated kinase 4 antibody
    • p21 Cdc42/Rac1-actiated kinase 4 antibody
    • P21 protein (Cdc42/Rac) activated kinase 4 antibody
    • p21(CDKN1A) activated kinase 4 antibody
    • p21-activated kinase 4 antibody
    • PAK 4 antibody
    • PAK-4 antibody
    • Pak4 antibody
    • PAK4_HUMAN antibody
    • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody
    • Serine threonine kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK4 antibody
    • Serine/threonine-protein kinase PAK 4 antibody
    see all

图片

  • Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/ml
    Lane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/ml

    All lanes : SW480 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • Immunohistochemistry of PAK4 in paraffin embedded human colon tissue section with ab62509 antibody at 10 µg/ml.
  • ICC/IF image of ab62509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of PAK4 in Human Colon cells using ab62509 at 20 ug/ml.

文献

This product has been referenced in:
  • Loganzo F  et al. Tumor cells chronically treated with a trastuzumab-maytansinoid antibody-drug conjugate develop varied resistance mechanisms but respond to alternate treatments. Mol Cancer Ther 14:952-63 (2015). Read more (PubMed: 25646013) »
  • Wen X  et al. ROCK1 and LIM Kinase Modulate Retrovirus Particle Release and Cell-Cell Transmission Events. J Virol 88:6906-21 (2014). Read more (PubMed: 24696479) »

See all 5 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas, islet cell tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 8.5
Permeabilization
No
Specification
Pancreas, islet cell tumor
Fixative
Formaldehyde
Username

Mr. Allan Wang

Verified customer

提交于 Apr 17 2018

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (SDS-PAGE 10%)
Sample
Chinese Hamster Cell lysate - whole cell (CHO cells)
Specification
CHO cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册