Anti-PAK1 + PAK2 + PAK3 (phospho T423)抗体(ab2477)

概述

  • 产品名称Anti-PAK1 + PAK2 + PAK3 (phospho T423)抗体
    参阅全部 PAK1 + PAK2 + PAK3 一抗
  • 描述
    兔多克隆抗体to PAK1 + PAK2 + PAK3 (phospho T423)
  • 特异性This phospho specific polyclonal antibody is specific for phosphorylated Threonine 423 of Human PAK 1 + 2 + 3. Reactivity with non-phosphorylated PAK 1 + 2 + 3 is less than 1% by ELISA.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: EQSKRSTpMVGTPY conjugated to KLH, corresponding to amino acids 413-433 of Human PAK 1, 408-428 of human PAK 2 and 411-431 of human PAK 3.

  • 常规说明


    P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • Primary antibody说明P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/4000 - 1/20000.
WB 1/500 - 1/2000. Detects a band of approximately 65 kDa.

靶标

  • 相关性In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
  • 细胞定位Cytoplasm. Recruited to focal adhesions upon activation
  • 数据库链接
  • 别名
    • Alpha PAK antibody
    • Beta PAK antibody
    • bPAK antibody
    • CDKN1A antibody
    • Gamma PAK antibody
    • hPAK3 antibody
    • MRX30 antibody
    • MRX47 antibody
    • Oligophrenin 3 antibody
    • OPHN3 antibody
    • P21 (CDKN1A) activated kinase 2 antibody
    • P21 (CDKN1A) activated kinase 3 antibody
    • p21 activated kinase 1 antibody
    • p21 activated kinase 2 antibody
    • p21 activated kinase 3 antibody
    • P21 protein (Cdc42/Rac) activated kinase 1 antibody
    • P21 protein (Cdc42/Rac) activated kinase 2 antibody
    • P21 protein (Cdc42/Rac) activated kinase 3 antibody
    • P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast) antibody
    • P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • P58 antibody
    • P65 PAK antibody
    • PAK 2 antibody
    • PAK 3 antibody
    • PAK1 antibody
    • PAK2 antibody
    • PAK3 antibody
    • PAK3beta antibody
    • PAK65 antibody
    • PAKalpha antibody
    • PAKgamma antibody
    • S6/H4 kinase antibody
    • Serine/threonine protein kinase PAK 1 antibody
    • Serine/threonine protein kinase PAK 2 antibody
    • Serine/threonine protein kinase PAK 3 antibody
    • Serine/threonine protein kinase PAK1 antibody
    • Serine/threonine protein kinase PAK2 antibody
    • Serine/threonine protein kinase PAK3 antibody
    • STE20 homolog, yeast antibody
    see all

Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody 图像

  • Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.

Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)参考文献

This product has been referenced in:
  • Gadepalli R  et al. Novel Role for p21-activated Kinase 2 in Thrombin-induced Monocyte Migration. J Biol Chem 288:30815-31 (2013). WB . Read more (PubMed: 24025335) »

See 1 Publication for this product

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