Anti-PAK1 + PAK2 + PAK3 (phospho T402)抗体(ab30577)

概述

  • 产品名称
    Anti-PAK1 + PAK2 + PAK3 (phospho T402)抗体
    参阅全部 PAK1 + PAK2 + PAK3 一抗
  • 描述
    兔多克隆抗体to PAK1 + PAK2 + PAK3 (phospho T402)
  • 特异性
    ab30577 recognises PAK1 + PAK2 + PAK3 (phospho T402).
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic Phosphopeptide corresponding to amino acid residues surrounding the phospho Thr402 of rat p21 Activated Kinase 2 (PAK2). The peptide sequence used is identical in PAK1, 2 and 3.

  • 阳性对照
    • Rat hippocampal lysate
  • 常规说明
    Note: Thr402 in PAK2 corresponds to Thr423 in human PAK1.


    Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK.

性能

应用

Our Abpromise guarantee covers the use of ab30577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa).

靶标

图片

  • All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577) at 1/1000 dilution

    Lane 1 : untreated rat hippocampal lysate
    Lane 2 : rat hippocampal lysate, pretreated with 1200 units lambda Ptase for 30 min (negative control)


    Predicted band size : 60 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab30577 has not yet been referenced specifically in any publications.

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