Anti-PAK1 + PAK2 + PAK3 (phospho S141)抗体(ab5247)

概述

  • 产品名称Anti-PAK1 + PAK2 + PAK3 (phospho S141)抗体
    参阅全部 PAK1 + PAK2 + PAK3 一抗
  • 描述
    兔多克隆抗体to PAK1 + PAK2 + PAK3 (phospho S141)
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit
  • 免疫原

    Chemically synthesized phosphopeptide derived from a region of human PAK2 that contains serine 141. The sequence is conserved in rat and rabbit.

  • 阳性对照
    • Cos cells transiently transfected with PAK 1, PAK 2, or PAK 3, and co transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation.
  • 常规说明


    P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PAK. The final product is generated by affinity chromatography using a PAK 2 derived peptide that is phosphorylated at serine 141.
  • Primary antibody说明P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 65 kDa.

靶标

Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody 图像



  • Predicted band size : 65 kDa


    Induction of Activation: Extracts prepared from Cos cells transiently transfected with PAK 1 and V12 Cdc42, and kept in suspension (inactive) or as adherent cultures (active) were resolved by SDSPAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either not treated or treated with lambda phosphatase, blocked with a 5% BSA-TBST buffer overnight at 4°C, and then incubated with 0.50 µg/mL ab5247 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.


  • Predicted band size : 65 kDa


    Peptide Competition: Extracts prepared from Cos cells transiently transfected with PAK 1 (lanes 1-4), PAK 2 (lanes 5-8), or PAK 3 (lanes 9-12) and cotransfected with V12 Cdc42 to induce activation were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5247 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5, 9), the nonphosphopeptide corresponding to the immunogen (2, 6, 10), a generic phosphoserine containing peptide (3, 7, 11), or, the phosphopeptide immunogen (4, 8, 12). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that the ab5247 antibody is highly selective for the phosphorylated form of PAK, and that the antibody can be used to detect an active form of PAK 1 as well as PAK 2 and PAK 3.

Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)参考文献

This product has been referenced in:
  • Kuželová K  et al. Group I PAK inhibitor IPA-3 induces cell death and affects cell adhesivity to fibronectin in human hematopoietic cells. PLoS One 9:e92560 (2014). WB ; Human . Read more (PubMed: 24664099) »
  • Awasthi A  et al. Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes. J Exp Med 207:1923-38 (2010). WB ; Mouse . Read more (PubMed: 20733035) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab5247.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"