PAK1 overexpression 293T裂解液(全细胞) (ab94241)

概述

  • 产品名称PAK1 overexpression 293T裂解液(全细胞)
  • 描述
    PAK1 overexpression裂解液
  • 常规说明ab94241 is a 293T cell transfected lysate in which Human PAK1 has been transiently over-expressed using a pCMV-PAK1 Full length plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain. PTM: Autophosphorylated when activated by CDC42/p21 and RAC1.

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应用

Our Abpromise guarantee covers the use of ab94241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

PAK1 overexpression 293T lysate (whole cell) 图像

  • ab94241 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PAK1 antibody (ab58263) at 1/500 dilution

    Lane 1 : PAK1 overexpression 293T lysate (whole cell) (ab94241)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

PAK1 overexpression 293T lysate (whole cell) (ab94241)参考文献

ab94241 has not yet been referenced specifically in any publications.

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