The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).
Note: This product requires purification with Protein A prior to ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性PAK1 interacting protein 1 (PAK1IP1) negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which have been shown to play a positive role in the regulation of signaling pathways involving MAPK8 and RELA. PAK1 exists as an inactive homodimer, which is activated by binding of small GTPases such as CDC42 to an N terminal regulatory domain. PAK1IP1 also binds to the N terminus of PAK1, and inhibits the specific activation of PAK1 by CDC42.