概述

  • 产品名称Anti-PAK1抗体
    参阅全部 PAK1 一抗
  • 描述
    兔多克隆抗体to PAK1
  • 特异性Detects endogenous levels of total PAK1.
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from Human PAK1 around the phosphorylation site of serine 199 (T-K-S-V-Y)

  • 阳性对照
    • K562 cells extracts; Human placenta tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab79218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

靶标

  • 功能The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated when activated by CDC42/p21 and RAC1.
  • 细胞定位Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Anti-PAK1 antibody 图像

  • Left panel: immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/50 ab79218; Right panel: same, after pre-incubation with the immunizing peptide.
  • All lanes : Anti-PAK1 antibody (ab79218) at 1/500 dilution

    Lane 1 : K562 cells extract
    Lane 2 : K562 cells extract with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 61 kDa

Anti-PAK1 antibody (ab79218)参考文献

ab79218 has not yet been referenced specifically in any publications.

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