The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).
功能The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain.
翻译后修饰Autophosphorylated when activated by CDC42/p21 and RAC1.
细胞定位Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
ab71549, at 1/10 dilution, staining PAK1 in human colon carcinoma by immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by a peroxidase-conjugated secondary antibody and DAB staining.
Anti-PAK1 antibody (ab71549)参考文献
has not yet been referenced specifically in any publications.