概述

  • 产品名称Anti-PAK1抗体
    参阅全部 PAK1 一抗
  • 描述
    兔多克隆抗体to PAK1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    KPLSSLTPLIAAAKEATKNNH

    , corresponding to amino acids 525-544 of Rat PAK1

  • 阳性对照
    • Brain extracts. Breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab32614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: 1/50. Perform heat mediated antigen retrieval by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min before commencing with IHC staining protocol.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 65 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated when activated by CDC42/p21 and RAC1.
    • 细胞定位Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ADRB2 antibody
      • Alpha PAK antibody
      • Alpha-PAK antibody
      • MGC130000 antibody
      • MGC130001 antibody
      • p21 activated kinase 1 antibody
      • p21 protein (Cdc42/Rac) activated kinase 1 antibody
      • p21-activated kinase 1 antibody
      • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
      • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
      • p65 PAK antibody
      • p65-PAK antibody
      • P68-PAK antibody
      • PAK alpha antibody
      • PAK-1 antibody
      • Pak1 antibody
      • PAK1_HUMAN antibody
      • Paka antibody
      • PAKalpha antibody
      • Protein kinase MUK2 antibody
      • Rac/p21-activated kinase antibody
      • Serine/threonine-protein kinase PAK 1 antibody
      • STE20 homolog yeast antibody
      see all

    Anti-PAK1 antibody 图像

    • Formalin fixed, paraffin embedded human breast carcinoma stained with ab32614 at 1/50.

    Anti-PAK1 antibody (ab32614)参考文献

    ab32614 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"