Anti-PAK1抗体(ab2551)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to PAK1
  • 经测试应用
    适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    E.coli expressed human PAK 1 (amino acids 333-545).

  • 常规说明


    Human p21-GTPase-activated Protein Kinase 1 (PAK 1) is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase, PAK 1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK 1 inhibits myosin light chain kinase, and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK 1 induces neurite outgrowth. PAK 1 may also regulate NFkappaB activation.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    Human p21-GTPase-activated Protein Kinase 1 (PAK 1) is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase, PAK 1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK 1 inhibits myosin light chain kinase, and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK 1 induces neurite outgrowth. PAK 1 may also regulate NFkappaB activation.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).

靶标

  • 功能
    The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • 细胞定位
    Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Anti-PAK1 antibody (ab2551)参考文献

ab2551 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The immunogen has 96% homology with PAK2 and PAK3. Thus, it's likely the antibody will cross react with PAK2 & PAK3. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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