- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-PAIP2抗体
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描述兔多克隆抗体to PAIP2
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宿主Rabbit
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PAIP2.
(Peptide available as ab36582.)
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: Jurkat; A431; HEK293; SHSY-5Y.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab33455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 14.9 kDa). |
靶标
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功能Acts as a repressor in the regulation of translation initiation of poly(A)-containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. Displaces the interaction of PABPC1 with poly(A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly(A) RNP structure organization.
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序列相似性Belongs to the PAIP2 family.
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结构域Only the PABPC1-interacting motif-1 (PAM1) interferes with the binding of PABPC1 to poly(A) RNA and translation initiation.
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细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 416187 Chicken
- Entrez Gene: 536619 Cow
- Entrez Gene: 51247 Human
- Entrez Gene: 67869 Mouse
- Entrez Gene: 361309 Rat
- Omim: 605604 Human
- SwissProt: Q5ZJS6 Chicken
- SwissProt: Q3ZC67 Cow
see all -
别名
- PABP-interacting protein 2 antibody
- PAIP-2 antibody
- PAIP2 antibody
see all
图片
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Western blot - Anti-PAIP2 antibody (ab33455)All lanes : Anti-PAIP2 antibody (ab33455) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 14.9 kDa
Observed band size: 17 kDa (why is the actual band size different from the predicted?)
文献
This product has been referenced in:
- Tahiri-Alaoui A et al. Poly(A) binding protein 1 enhances cap-independent translation initiation of neurovirulence factor from avian herpesvirus. PLoS One 9:e114466 (2014). Read more (PubMed: 25503397) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"