概述

  • 产品名称
    Anti-PAI1抗体[I-3]
    参阅全部 PAI1 一抗
  • 描述
    小鼠单克隆抗体[I-3] to PAI1
  • 特异性
    ab82218 is specific for human PAI-1. No cross-reactivity is observed with PAI-2 as tested by ELISA and immunoblotting. No reactivity is observed with any other human plasma protein when tested by immunoblotting.
  • 经测试应用
    适用于: WB, IHC-Fr, Indirect ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Partialy purified human PAI-1

  • 表位
    ab82218 binds to an epitope consisting of amino acids 235-283

性能

应用

Our Abpromise guarantee covers the use of ab82218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-Fr Use at an assay dependent concentration.
Indirect ELISA Use at an assay dependent concentration.

靶标

  • 功能
    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 组织特异性
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 疾病相关
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 序列相似性
    Belongs to the serpin family.
  • 翻译后修饰
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

文献

This product has been referenced in:
  • Gopal S  et al. Estrogen regulates endothelial migration via plasminogen activator inhibitor (PAI-1). Mol Hum Reprod 18:410-6 (2012). WB ; Rat . Read more (PubMed: 22389473) »
  • Marchand A  et al. miR-421 and miR-30c inhibit SERPINE 1 gene expression in human endothelial cells. PLoS One 7:e44532 (2012). WB . Read more (PubMed: 22952991) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (T47D breast cancer cell)
Loading amount
40 µg
Specification
T47D breast cancer cell
Treatment
17 beta estradiol 10 nM Time course experiment
Gel Running Conditions
Reduced Denaturing (8)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. santhosh gopal

Verified customer

提交于 Jul 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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