概述

  • 产品名称Anti-PAI1抗体
    参阅全部 PAI1 一抗
  • 描述
    兔多克隆抗体to PAI1
  • 经测试应用适用于: ICC/IF, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Rat).

  • 阳性对照
    • This antibody gave a positive signal in ICC/IF in MEF1 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab7205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
ICC 1/200.
WB 1/1000.

靶标

  • 功能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 组织特异性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 疾病相关Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 序列相似性Belongs to the serpin family.
  • 翻译后修饰Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Anti-PAI1 antibody 图像

  • Anti-PAI1 antibody (ab7205) at 1/1000 dilution
  • ICC/IF image of ab7205 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7205, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PAI1 antibody (ab7205)参考文献

This product has been referenced in:
  • Stefos GC  et al. NGF upregulates the plasminogen activation inhibitor-1 in neurons via the Calcineurin/NFAT pathway and the down syndrome-related proteins DYRK1A and RCAN1 attenuate this effect. PLoS One 8:e67470 (2013). WB . Read more (PubMed: 23825664) »
  • Abel B  et al. N-terminal tyrosine phosphorylation of caveolin-2 negates anti-proliferative effect of transforming growth factor beta in endothelial cells. FEBS Lett 586:3317-23 (2012). Read more (PubMed: 22819829) »

See all 4 Publications for this product

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