概述

  • 产品名称Anti-PAI1抗体
    参阅全部 PAI1 一抗
  • 描述
    山羊多克隆抗体to PAI1
  • 特异性This antibody is expected to recognise both reported isoforms.
  • 经测试应用适用于: Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-GFKIDDKGMAPALRH

    , corresponding to Internal sequence amino acids 86-100 of Human PAI1

  • 阳性对照
    • Human duodenum, liver and lung lysates.

性能

应用

Our Abpromise guarantee covers the use of ab31280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 1.5 - 3 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 组织特异性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 疾病相关Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 序列相似性Belongs to the serpin family.
  • 翻译后修饰Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Anti-PAI1 antibody 图像

  • Anti-PAI1 antibody (ab31280) at 0.1 µg/ml + Human Liver lysate at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody.

    See Abreview

  • ab31280 (4µg/ml) staining of paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • ab31280 (1.5ug/ml) used as the reporter with a capture rabbit antibody (2.5ug/ml).

Anti-PAI1 antibody (ab31280)参考文献

This product has been referenced in:
  • Keates AC  et al. Helicobacter pylori infection stimulates plasminogen activator inhibitor 1 production by gastric epithelial cells. Infect Immun 76:3992-9 (2008). WB ; Human . Read more (PubMed: 18519558) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lung Epithelial cell (Beas2B))
Loading amount 20 µg
Specification Lung Epithelial cell (Beas2B)
Gel Running Conditions Reduced Denaturing (gel 8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Jul 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Treatment Homogenised in 1% SDS lysis buffer
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Dr. Rachel Barker

Verified customer

提交于 Dec 08 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (MDA-MB-231 Breast Cancer Cells, grown in mouse bon)
Specification MDA-MB-231 Breast Cancer Cells, grown in mouse bon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate EDTA
Permeabilization No
Blocking step Casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Jul 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Bone Marrow Stromal Cells (HS-5))
Loading amount 5 µg
Specification Human Bone Marrow Stromal Cells (HS-5)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step 1x Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"