概述

  • 产品名称Anti-PAI1抗体
    参阅全部 PAI1 一抗
  • 描述
    兔多克隆抗体to PAI1
  • 经测试应用适用于: IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length PAI1 protein (Mouse).

  • 阳性对照
    • Paraffin sections of lung and carotid.

性能

应用

Our Abpromise guarantee covers the use of ab28207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 18276785
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 1 - 10 µg/ml.

靶标

  • 功能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 组织特异性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 疾病相关Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 序列相似性Belongs to the serpin family.
  • 翻译后修饰Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Anti-PAI1 antibody 图像

  • ab28207 staining PAI1 in E18.5 mouse tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Subsequent to deparaffinization, the sections were hydrated, heated in 10mM citrate buffer (pH 6.0) and treated with 1% H2O2 in methanol for 10 min and blocked with 10% of normal serum. The primary antibody was incubated with sample at 4°C overnight.

Anti-PAI1 antibody (ab28207)参考文献

This product has been referenced in:
  • Achuta VS  et al. Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile X mice. J Neurosci 34:1916-23 (2014). Mouse . Read more (PubMed: 24478370) »
  • Alamo P  et al. Subcutaneous preconditioning increases invasion and metastatic dissemination in mouse colorectal cancer models. Dis Model Mech 7:387-96 (2014). IHC-P . Read more (PubMed: 24487410) »

See all 8 Publications for this product

Product Wall

Thanks for your enquiry and for providing the protocol and data.

To our knowledge, this antibody has not previously been tested for reactivity to human PAI-1. It may be there is no reactivity to the human protein. Do you happen to have any mo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"