Anti-PAI1抗体[1D5] (ab125687)

概述

  • 产品名称Anti-PAI1抗体[1D5]
    参阅全部 PAI1 一抗
  • 描述
    小鼠单克隆抗体[1D5] to PAI1
  • 经测试应用适用于: WB, ELISA, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant fragment of Human PAI1 expressed in E. Coli.

  • 阳性对照
    • Recombinant Human PAI1 protein (aa194-316); PAI1 (aa194-316)-hIgGFc transfected HEK293 cell lysate; Human lung cancer and kidney cancer tissues; NIH 3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab125687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 45 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/200 - 1/1000.

靶标

  • 功能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 组织特异性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 疾病相关Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 序列相似性Belongs to the serpin family.
  • 翻译后修饰Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Anti-PAI1 antibody [1D5] 图像

  • Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution + Human PAI1 recombinant protein (amino acids 194-316)

    Predicted band size : 45 kDa
  • All lanes : Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PAI1-hIgGFc transfected HEK293 cell lysate (aa194-316)


    Predicted band size : 45 kDa
  • ELISA using ab125687.
  • ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human lung cancer tissue by Immunohistochemistry followed by DAB staining.
  • ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human kidney cancer tissue by Immunohistochemistry followed by DAB staining.
  • ab125687 at 1/200 dilution staining PAI1 in NIH 3T3 cells by Flow Cytometry (green), negative control (red).

Anti-PAI1 antibody [1D5] (ab125687)参考文献

ab125687 has not yet been referenced specifically in any publications.

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The price for antibody ab125687 is €390 plus €39 shipping charges to Switzerland.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"