概述

  • 产品名称
    Anti-PAI1抗体[1D5]
    参阅全部 PAI1 一抗
  • 描述
    小鼠单克隆抗体[1D5] to PAI1
  • 经测试应用
    适用于: WB, ELISA, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Purified recombinant fragment of Human PAI1 expressed in E. Coli.

  • 阳性对照
    • Recombinant Human PAI1 protein (aa194-316); PAI1 (aa194-316)-hIgGFc transfected HEK293 cell lysate; Human lung cancer and kidney cancer tissues; NIH 3T3 cells.
  • 常规说明

    This product was changed from ascites to supernatant. Lot no’s high than GR201079-27 are from Tissue Culture Supernatant

性能

应用

Our Abpromise guarantee covers the use of ab125687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 45 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/200 - 1/1000.

靶标

  • 功能
    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • 组织特异性
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 疾病相关
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 序列相似性
    Belongs to the serpin family.
  • 翻译后修饰
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

图片

  • Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution + Human PAI1 recombinant protein (amino acids 194-316)

    Predicted band size : 45 kDa
  • All lanes : Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PAI1-hIgGFc transfected HEK293 cell lysate (aa194-316)


    Predicted band size : 45 kDa
  • ELISA using ab125687.
  • ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human lung cancer tissue by Immunohistochemistry followed by DAB staining.
  • ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human kidney cancer tissue by Immunohistochemistry followed by DAB staining.
  • ab125687 at 1/200 dilution staining PAI1 in NIH 3T3 cells by Flow Cytometry (green), negative control (red).

文献

This product has been referenced in:
  • Li Y  et al. LPS induces HUVEC angiogenesis in vitro through miR-146a-mediated TGF-ß1 inhibition. Am J Transl Res 9:591-600 (2017). WB ; Human . Read more (PubMed: 28337286) »
  • Liang Q  et al. Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice. Iran J Basic Med Sci 19:43-8 (2016). WB ; Mouse . Read more (PubMed: 27096063) »

See all 3 Publications for this product

客户评价及客户问答

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The price for antibody ab125687 is €390 plus €39 shipping charges to Switzerland.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or ...

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Thank you for your reply.

As ab125687 PAI1 antibody [1D5], ab1834 Factor XIIIa antibody [AC-1A1] and ab54488 CD41/Integrin alpha 2b antibody [283.16B7] are all mouse IgG1, Ican suggest to consider the following anti mouse IgG1 secondaries av...

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Merci de votre question et votre intérêt pour nos produits.

L'anticorps secondaire ab98689 n'est pas couplé à la biotine - c'est juste l'anticorps secondaire non-conjugué. Les anticorps pré-adsorb&eacu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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