概述

  • 产品名称Anti-PAG抗体[PAG-C1]
    参阅全部 PAG 一抗
  • 描述
    小鼠单克隆抗体[PAG-C1] to PAG
  • 经测试应用适用于: IHC-P, IP, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic C-teminal peptide (last 15 amino acids) (Human) conjugated to KLH.

  • 表位Located in the C-terminal domain.
  • 阳性对照
    • Human Ramos cell line, Raji cell line Mouse spleen cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from hybridoma culture supernatant by protein A affinity chromatography.
  • 克隆单克隆
  • 克隆编号PAG-C1
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Use covalently bound ab4206 to Sepharose beads as immunosorbent. This method of precipitation obviously differs from protein A/G-sepharose or Goat xMs Sepharose mediated immunoprecipitation protocols.

WB Use at an assay dependent concentration.

This antibody can be used for WB, but the signal is very faint, and we do not recommend or guarantee it for this method. We recommend the clone MEM-255 (ab9527) or the rabbit polyclonal ab14989, which are much better for WB.

Flow Cyt Use 1µg for 106 cells.

靶标

  • 功能Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
  • 组织特异性Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
  • 翻译后修饰Palmitoylated.
    Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
  • 细胞定位Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBP antibody
    • Csk binding protein antibody
    • Csk-binding protein antibody
    • FLJ37858 antibody
    • MGC138364 antibody
    • PAG 1 antibody
    • PAG antibody
    • PAG1 antibody
    • PAG1_HUMAN antibody
    • Phosphoprotein associated with glycosphingolipid enriched microdomains 1 antibody
    • Phosphoprotein associated with glycosphingolipid enriched microdomains antibody
    • Phosphoprotein associated with glycosphingolipid microdomains 1 antibody
    • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 antibody
    • Protein Associated with Glycosphingolipid Enriched Microdomains antibody
    • Transmembrane adapter protein PAG antibody
    • Transmembrane phosphoprotein Cbp antibody
    see all

Anti-PAG antibody [PAG-C1] 图像

  • Overlay histogram showing Jurkat cells stained with ab4206 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4206, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • 1. Frozen Raji cells lysate, immunoprecipitated with ab4206

    2. Fresh Raji cells lysate, immunoprecipitated with ab4206

    3. Frozen Raji cells lysate, immunoprecipitated with ab14989

    Reprobe with ab9527.

    The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.

  • ab4206 staining PAG in human germinal center of lymphatic follicle and in dispersed T cells in appendix tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in citrate buffer pH 6.1. Th image show positive signal in T-cells.

Anti-PAG antibody [PAG-C1] (ab4206)参考文献

This product has been referenced in:
  • Baumgartner M  et al. Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes. Blood 101:1874-81 (2003). Read more (PubMed: 12411311) »
  • Vang T  et al. Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem 278:17597-600 (2003). Read more (PubMed: 12665526) »

See all 2 Publications for this product

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