Anti-PAG抗体[MEM-255] (ab9527)

概述

  • 产品名称Anti-PAG抗体[MEM-255]
    参阅全部 PAG 一抗
  • 描述
    小鼠单克隆抗体[MEM-255] to PAG
  • 特异性This antibody is specific for CSK Binding Protein
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human CSK Binding Protein.

  • 阳性对照
    • RAMOS and Jurkat cells
  • 常规说明Csk-Binding Protein is also known as PAG (Protein Associated with Glycosphingolipid-enriched microdomains).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by protein A-affinity chromatography.
  • 克隆单克隆
  • 克隆编号MEM-255
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at an assay dependant dilution. Use a lysis buffer made with Tris HCl (pH 8.1) and 1%laurylmaltoside (with common protease inhibitors). Use under non-reducing conditions.
    Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. The reason is, that PAG has a pI value of about 4.6 and binds poorely SDS and therefore it had decreased mobility on SDS gels.

    Is unsuitable for IP (use ab4206 or ab14989).

    May be unsuitable for ICC-IF due to unsuccesful results in submitted Abreview. (Please see Abreview comments)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
    • 组织特异性Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
    • 翻译后修饰Palmitoylated.
      Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
    • 细胞定位Cell membrane. Present in lipid rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CBP antibody
      • Csk binding protein antibody
      • Csk-binding protein antibody
      • FLJ37858 antibody
      • MGC138364 antibody
      • PAG 1 antibody
      • PAG antibody
      • PAG1 antibody
      • PAG1_HUMAN antibody
      • Phosphoprotein associated with glycosphingolipid enriched microdomains 1 antibody
      • Phosphoprotein associated with glycosphingolipid enriched microdomains antibody
      • Phosphoprotein associated with glycosphingolipid microdomains 1 antibody
      • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 antibody
      • Protein Associated with Glycosphingolipid Enriched Microdomains antibody
      • Transmembrane adapter protein PAG antibody
      • Transmembrane phosphoprotein Cbp antibody
      see all

    Anti-PAG antibody [MEM-255] (ab9527)参考文献

    This product has been referenced in:
    • Mutch CM  et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). Read more (PubMed: 17090619) »
    • Brdickova N  et al. Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett 507:133-6 (2001). Read more (PubMed: 11684085) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa cells)
    Specification HeLa cells
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    提交于 Mar 22 2007

    Thank you for your enquiry. CSK Binding Protein antibody Ref:ab9527 [MEM-255] should stain blots strongly in dilutions 1:500 - 1:1000 but optimum dilution should be done for a particular system of a researcher. We did not measure protein load, it alway...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"