Anti-PAF49抗体[MA34] (ab111161)

概述

  • 产品名称Anti-PAF49抗体[MA34]
    参阅全部 PAF49 一抗
  • 描述
    小鼠单克隆抗体[MA34] to PAF49
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    PAF49 isolated from HeLa cells.

  • 阳性对照
    • Purified PAF49 protein.

性能

应用

Our Abpromise guarantee covers the use of ab111161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/10 - 1/100.
ICC/IF 1/100 - 1/1000.

靶标

  • 功能DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
    Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
  • 序列相似性Belongs to the eukaryotic RPA34 RNA polymerase subunit family.
  • 翻译后修饰Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
    Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A34.5 antibody
    • Anti sense to ERCC 1 protein antibody
    • Antisense to ERCC-1 protein antibody
    • ASE 1 antibody
    • ASE-1 antibody
    • ASE1 antibody
    • CAST antibody
    • CD3 epsilon associated protein antibody
    • CD3-epsilon-associated protein antibody
    • CD3E antigen, epsilon polypeptide associated protein antibody
    • CD3E associated protein antibody
    • CD3e molecule, epsilon associated protein antibody
    • CD3E-associated protein antibody
    • CD3EAP antibody
    • DNA directed RNA polymerase I subunit RPA34 antibody
    • DNA-directed RNA polymerase I subunit RPA34 antibody
    • MGC118851 antibody
    • PAF 49 antibody
    • RNA polymerase I associated factor PAF49 antibody
    • RNA polymerase I-associated factor PAF49 antibody
    • RPA34_HUMAN antibody
    see all

Anti-PAF49 antibody [MA34] 图像

  • Immunofluorescent analysis of PAF49 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PAF49 monoclonal antibody (ab111161) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. PAF49 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunofluorescent analysis of PAF49 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PAF49 monoclonal antibody (ab111161) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. PAF49 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunofluorescent analysis of PAF49 in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PAF49 monoclonal antibody (ab111161) at a dilution of 1:200 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. PAF49 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a PAF49 monoclonal antibody (ab111161) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a PAF49 monoclonal antibody (ab111161) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a PAF49 monoclonal antibody (ab111161) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Anti-PAF49 antibody [MA34] (ab111161) at 1/500 dilution + Purified PAF49 protein

    Predicted band size : 55 kDa

Anti-PAF49 antibody [MA34] (ab111161)参考文献

ab111161 has not yet been referenced specifically in any publications.

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