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MEPHDSSHMDSEFRYTLconjugated to KLH, corresponding to N terminal amino acids 1-17 of Human PAF Receptor (Peptide available as ab107706.)
Our Abpromise guarantee covers the use of ab104162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Predicted molecular weight: 39 kDa.Can be blocked with PAF Receptor peptide (ab107706).|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
PAF Receptor was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to PAF Receptor and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104162.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 39kDa; PAF Receptor
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