Anti-PADI4 / PAD4抗体(ab50247)


  • 产品名称Anti-PADI4 / PAD4抗体
    参阅全部 PADI4 / PAD4 一抗
  • 描述
    兔多克隆抗体to PADI4 / PAD4
  • 特异性This antibody reacts specifically with the 74 kDa PADI4 / PAD4 protein. It shows no cross reactivity with the other isoforms.
  • 经测试应用适用于: WB, IP, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A 15 amino acid synthetic peptide derived from the C-terminal domain of human PADI4/ PAD4.



Our Abpromise guarantee covers the use of ab50247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/2000. Predicted molecular weight: 74 kDa.Can be blocked with PADI4 / PAD4 peptide (ab156172).
IP 1/20 - 1/100.
ELISA 1/1000 - 1/5000.
ICC/IF 1/200.


  • 功能Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • 组织特异性Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
  • 疾病相关Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
  • 序列相似性Belongs to the protein arginine deiminase family.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC antibody
    • HL 60 PAD antibody
    • HL-60 PAD antibody
    • PAD 4 antibody
    • PAD antibody
    • PADI 4 antibody
    • PADI 5 antibody
    • PADI H protein antibody
    • Padi4 antibody
    • PADI4_HUMAN antibody
    • PADI5 antibody
    • PDI 4 antibody
    • PDI 5 antibody
    • PDI4 antibody
    • PDI5 antibody
    • Peptidyl arginine deiminase type IV antibody
    • Peptidyl arginine deiminase type V antibody
    • Peptidylarginine deiminase IV antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 4 antibody
    • Protein arginine deiminase type IV antibody
    • Protein-arginine deiminase type IV antibody
    • Protein-arginine deiminase type-4 antibody
    see all

Anti-PADI4 / PAD4 antibody 图像

  • ICC/IF image of ab50247 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50247, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PADI4 / PAD4 antibody (ab50247)参考文献

This product has been referenced in:
  • Sylvestersen KB  et al. Proteomic analysis of arginine methylation sites in human cells reveals dynamic regulation during transcriptional arrest. Mol Cell Proteomics 13:2072-88 (2014). Read more (PubMed: 24563534) »

See 1 Publication for this product

Product Wall

The PAD4 protein is predicted to run around 74 kDa based on the molecular weight of the amino acid sequence, however full length PAD4 has been shown to migrate around 67 kDa in SDS-PAGE:

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I am writing to inform you that the blocking peptide for ab50247 has now been added to our catalog. The product id is PADI4 / PAD4 peptide (ab156172). The datasheet for this product may be found at:

Thank you for contacting us.

The exact concentration is necessary when you would like to use Isotype control in IHC or Immunoprecipitation other than that it is OK.

Please note that we can only provide exact concentration for ab128089...

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Thank you for your reply.

There are a few variations that may work:

1 - Depending on the blocking agent you are using, you could try using 5% BSA, as this may help reduce the background levels that you are seeing.

2 - If y...

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Thank you for contacting Abcam.

If you could provide me with the protocol that you are using, I would be happy to see if there are any protocol suggestions I can make that may help resolve some of the issues we are having.

Also we h...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Lung)
Specification Lung
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C

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提交于 Apr 25 2011