Anti-PADI2 / PAD2抗体(ab50257)


  • 产品名称Anti-PADI2 / PAD2抗体
    参阅全部 PADI2 / PAD2 一抗
  • 描述
    兔多克隆抗体to PADI2 / PAD2
  • 特异性This antibody reacts specifically with the 76 kDa PADI2 / PAD2 protein. It shows no cross reactivity with the other isoforms.
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 15 amino acid synthetic peptide derived from the C-terminal domain of human PADI2/ PAD2.



Our Abpromise guarantee covers the use of ab50257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/200. Predicted molecular weight: 76 kDa.


  • 功能Catalyzes the deimination of arginine residues of proteins.
  • 序列相似性Belongs to the protein arginine deiminase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0994 antibody
    • OTTHUMP00000044625 antibody
    • PAD 2 antibody
    • PAD H19 antibody
    • PAD-H19 antibody
    • PAD2 antibody
    • PADI 2 antibody
    • Padi2 antibody
    • PADI2 protein antibody
    • PADI2_HUMAN antibody
    • PDI 2 antibody
    • PDI2 antibody
    • Peptidlyarginine deiminase type II antibody
    • Peptidyl arginine deiminase II antibody
    • Peptidyl arginine deiminase type II antibody
    • Peptidylarginine deiminase II antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 2 antibody
    • Protein arginine deiminase type II antibody
    • Protein-arginine deiminase type II antibody
    • Protein-arginine deiminase type-2 antibody
    see all

Anti-PADI2 / PAD2 antibody 图像

  • ICC/IF image of ab50257 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50257, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab50257 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50257, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PADI2 / PAD2 antibody (ab50257)参考文献

This product has been referenced in:
  • Chang HH  et al. A molecular signature of preclinical rheumatoid arthritis triggered by dysregulated PTPN22. JCI Insight 1:e90045 (2016). WB ; Human . Read more (PubMed: 27777982) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Hepatocytes)
Specification Hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Dec 26 2014

Thank you for your reply.

The stock is roughly 5 mg/ml (milligrams per milliliter). You need to use 5 ug/ml (micrograms per millileter) for the ICC staining. Start with a 1/1000 dilution, but final optimization may be necessary dependin...

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Thank you for your inquiry.

Since this antibody is a whole antiserum, it is not purified and a concentration has not been determined. But generally, for this format, you can estimate the total protein concentration between 1-10 mg/ml. I...

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