Anti-PADI2 / PAD2抗体(ab16478)

概述

  • 产品名称
    Anti-PADI2 / PAD2抗体
    参阅全部 PADI2 / PAD2 一抗
  • 描述
    兔多克隆抗体to PADI2 / PAD2
  • 特异性
    ab16478 recognises a specific 43kDa band corresponding to PADI2, which is specifically blocked using the immunizing peptide in human colon, skeletal muscle and kidney lysates. There is a non-specific 18kDa band present in skeletal muscle lysates, which is attributed to cross-reactivity of the PADI2 antibody
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PADI2/ PAD2.

  • 阳性对照
    • This antibody gave a positive signal in the following whole tissue lysates: Human Kidney Normal.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Rectum Normal.

      This antibody gave a positive signal in the following cell lines: HEK293; Human Peripheral Blood Mononuclear cells.

性能

应用

Our Abpromise guarantee covers the use of ab16478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. PubMed: 19085382
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa).

靶标

  • 功能
    Catalyzes the deimination of arginine residues of proteins.
  • 序列相似性
    Belongs to the protein arginine deiminase family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0994 antibody
    • OTTHUMP00000044625 antibody
    • PAD 2 antibody
    • PAD H19 antibody
    • PAD-H19 antibody
    • PAD2 antibody
    • PADI 2 antibody
    • Padi2 antibody
    • PADI2 protein antibody
    • PADI2_HUMAN antibody
    • PDI 2 antibody
    • PDI2 antibody
    • Peptidlyarginine deiminase type II antibody
    • Peptidyl arginine deiminase II antibody
    • Peptidyl arginine deiminase type II antibody
    • Peptidylarginine deiminase II antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 2 antibody
    • Protein arginine deiminase type II antibody
    • Protein-arginine deiminase type II antibody
    • Protein-arginine deiminase type-2 antibody
    see all

图片

  • Image courtesy of Human Protein Atlas

    ab16478 staining PADI2 in female rectum, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human rectal tissue was incubated with ab16478 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab16478 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab16478 staining human peripheral blood mononuclear cells (cultured with M-CSF) by Flow Cytometery. Cells were treated with flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) and gating was done on myeloid cells. The primary antibody was diluted 1/10 (PBS 0.1% sodium azide 1% BSA) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

文献

This product has been referenced in:
  • Turunen S  et al. Rheumatoid arthritis antigens homocitrulline and citrulline are generated by local myeloperoxidase and peptidyl arginine deiminases 2, 3 and 4 in rheumatoid nodule and synovial tissue. Arthritis Res Ther 18:239 (2016). IHC-P ; Human . Read more (PubMed: 27765067) »
  • Jagessar SA  et al. Lymphocryptovirus Infection of Nonhuman Primate B Cells Converts Destructive into Productive Processing of the Pathogenic CD8 T Cell Epitope in Myelin Oligodendrocyte Glycoprotein. J Immunol 197:1074-88 (2016). ICC/IF ; Monkey . Read more (PubMed: 27412414) »

See all 8 Publications for this product

客户评价及客户问答

Vielen Dank für Ihre Email.

Leider weiss ich auch nicht genau, was der Unterschied zwischen diesen beiden Banden ist. In UniProt hat es zwei Sequenzen - die annotierte lange Form (http://www.uniprot.org/uniprot/Q9Y2J8) und die noch un- a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Peripheral blood mononuclear cells)
Specification
Peripheral blood mononuclear cells
Preparation
Cell harvesting/tissue preparation method: Normal human peripheral blood monocytes cultured with M-CSF. Cells were harvested on day 3, non-specific binding was blocked with human Ig, staining was performed on permeabilized cells
Sample buffer: flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) after permeabilization
Fixation
Caltag Fix Perm Kit
Permeabilization
Yes - Caltag Fix Perm Kit
Gating Strategy
Myeloid gate
Username

Dr. Frances Santiago-Schwarz

Verified customer

提交于 Jul 01 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1, MRC-5, human macrophages)
Loading amount
1e+006 cells
Specification
THP-1, MRC-5, human macrophages
Blocking step
Other as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Oct 10 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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