Anti-PADI2 / PAD2抗体(ab16478)

概述

  • 产品名称Anti-PADI2 / PAD2抗体
    参阅全部 PADI2 / PAD2 一抗
  • 描述
    兔多克隆抗体to PADI2 / PAD2
  • 特异性ab16478 recognises a specific 43kDa band corresponding to PADI2, which is specifically blocked using the immunizing peptide in human colon, skeletal muscle and kidney lysates. There is a non-specific 18kDa band present in skeletal muscle lysates, which is attributed to cross-reactivity of the PADI2 antibody
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PADI2/ PAD2.

  • 阳性对照
    • This antibody gave a positive signal in the following whole tissue lysates: Human Kidney Normal.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Rectum Normal.

      This antibody gave a positive signal in the following cell lines: HEK293; Human Peripheral Blood Mononuclear cells.

性能

应用

Our Abpromise guarantee covers the use of ab16478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. PubMed: 19085382
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 76 kDa).

靶标

  • 功能Catalyzes the deimination of arginine residues of proteins.
  • 序列相似性Belongs to the protein arginine deiminase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0994 antibody
    • OTTHUMP00000044625 antibody
    • PAD 2 antibody
    • PAD H19 antibody
    • PAD-H19 antibody
    • PAD2 antibody
    • PADI 2 antibody
    • Padi2 antibody
    • PADI2 protein antibody
    • PADI2_HUMAN antibody
    • PDI 2 antibody
    • PDI2 antibody
    • Peptidlyarginine deiminase type II antibody
    • Peptidyl arginine deiminase II antibody
    • Peptidyl arginine deiminase type II antibody
    • Peptidylarginine deiminase II antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 2 antibody
    • Protein arginine deiminase type II antibody
    • Protein-arginine deiminase type II antibody
    • Protein-arginine deiminase type-2 antibody
    see all

Anti-PADI2 / PAD2 antibody 图像

  • Image courtesy of Human Protein Atlas

    ab16478 staining PADI2 in female rectum, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human rectal tissue was incubated with ab16478 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab16478 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • ab16478 staining human peripheral blood mononuclear cells (cultured with M-CSF) by Flow Cytometery. Cells were treated with flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) and gating was done on myeloid cells. The primary antibody was diluted 1/10 (PBS 0.1% sodium azide 1% BSA) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-PADI2 / PAD2 antibody (ab16478)参考文献

This product has been referenced in:
  • Chang X  et al. PADI2 is significantly associated with rheumatoid arthritis. PLoS One 8:e81259 (2013). WB ; Human . Read more (PubMed: 24339914) »
  • Kilsgård O  et al. Peptidylarginine Deiminases Present in the Airways During Tobacco Smoking and Inflammation can Citrullinate the Host Defense Peptide LL-37 Resulting in Altered Activities. Am J Respir Cell Mol Biol : (2011). IHC-P ; Human . Read more (PubMed: 21960546) »

See all 5 Publications for this product

Product Wall

Vielen Dank für Ihre Email.

Leider weiss ich auch nicht genau, was der Unterschied zwischen diesen beiden Banden ist. In UniProt hat es zwei Sequenzen - die annotierte lange Form (http://www.uniprot.org/uniprot/Q9Y2J8) und die noch un- a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Peripheral blood mononuclear cells)
Specification Peripheral blood mononuclear cells
Preparation Cell harvesting/tissue preparation method: Normal human peripheral blood monocytes cultured with M-CSF. Cells were harvested on day 3, non-specific binding was blocked with human Ig, staining was performed on permeabilized cells
Sample buffer: flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) after permeabilization
Fixation Caltag Fix Perm Kit
Permeabilization Yes - Caltag Fix Perm Kit
Gating Strategy Myeloid gate
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Dr. Frances Santiago-Schwarz

Verified customer

提交于 Jul 01 2009

Application Western blot
Sample Human Cell lysate - whole cell (THP-1, MRC-5, human macrophages)
Loading amount 1e+006 cells
Specification THP-1, MRC-5, human macrophages
Blocking step Other as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

提交于 Oct 10 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"