Anti-PACT (PKR activating protein) / PRKRA抗体(ab82364)

概述

  • 产品名称Anti-PACT (PKR activating protein) / PRKRA抗体
    参阅全部 PACT (PKR activating protein) / PRKRA 一抗
  • 描述
    兔多克隆抗体to PACT (PKR activating protein) / PRKRA
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide selected from S246 region of Human PACT (PKR activating protein)/ PRKRA conjugated to KLH (NP_003681)

  • 阳性对照
    • HL60 cell line lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab82364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ELISA 1/1000.

靶标

  • 功能Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.
  • 疾病相关Defects in PRKRA are the cause of dystonia type 16 (DYT16) [MIM:612067]. DYT16 is an early-onset dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT16 patients have progressive, generalized dystonia with axial muscle involvement, oro-mandibular (sardonic smile) and laryngeal dystonia and, in some cases, parkinsonian features.
  • 序列相似性Belongs to the PRKRA family.
    Contains 3 DRBM (double-stranded RNA-binding) domains.
  • 结构域Self-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3.
  • 翻译后修饰Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DYT16 antibody
    • HSD14 antibody
    • Interferon inducible double stranded RNA dependent protein kinase activator A antibody
    • interferon-inducible double stranded RNA-dependent activator antibody
    • Interferon-inducible double stranded RNA-dependent protein kinase activator A antibody
    • PACT antibody
    • PKR associated protein X antibody
    • PKR associating protein X antibody
    • PKR-associated protein X antibody
    • PKR-associating protein X antibody
    • PRKRA antibody
    • PRKRA_HUMAN antibody
    • Protein activator of the interferon induced protein kinase antibody
    • Protein activator of the interferon-induced protein kinase antibody
    • Protein kinase antibody
    • Protein kinase interferon inducible double stranded RNA dependent activator antibody
    • RAX antibody
    see all

Anti-PACT (PKR activating protein) / PRKRA antibody 图像

  • Anti-PACT (PKR activating protein) / PRKRA antibody (ab82364) at 1/50 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

Anti-PACT (PKR activating protein) / PRKRA antibody (ab82364)参考文献

ab82364 has not yet been referenced specifically in any publications.

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