Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 325 and 375 of Human PACSIN3 (NP_057307.2)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
Use at 2-5 µg/mg of lysate.
May play a role in vesicle formation and transport.
Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
Belongs to the PACSIN family.
Contains 1 FCH domain. Contains 1 SH3 domain.
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Cytoplasm. Cell membrane. Detected at the inner aspect of the plasma membrane in cells or myotubes.
Information by UniProt
Endophilin 1 antibody
Endophilin I antibody
Western blot - Anti-PACSIN3 antibody (ab86077)
All lanes : Anti-PACSIN3 antibody (ab86077) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Predicted band size: 47 kDa Detection: chemiluminescence with exposure time of 30 seconds
Immunoprecipitation - Anti-PACSIN3 antibody (ab86077)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86077.
20% of IP was loaded per lane, and probed with ab86077 at 1µg/ml (lane 1) or with a control IgG (lane 2).
Detection: chemiluminescence with an exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
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