Anti-PACE4抗体(ab110144)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to PACE4
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    PDCEPGTYFDSE

    , corresponding to internal sequence amino acids 830-841 of Human PACE4 (NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1).

  • 阳性对照
    • Human testis and skeletal muscle tissues

性能

应用

Our Abpromise guarantee covers the use of ab110144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200. Fix cells in paraformaldehyde and permeabilize with 0.5% Triton X-100/PBS before staining.
IHC-P Use a concentration of 3.75 µg/ml.

靶标

  • 功能
    Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
  • 组织特异性
    Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
  • 序列相似性
    Belongs to the peptidase S8 family.
    Contains 1 homo B/P domain.
    Contains 1 PLAC domain.
  • 结构域
    The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
  • 细胞定位
    Secreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Paired basic amino acid cleaving enzyme 4 antibody
    • Paired basic amino acid cleaving system 4 antibody
    • PCSK6 antibody
    • PCSK6_HUMAN antibody
    • Proprotein convertase subtilisin/kexin type 6 antibody
    • SPC4 antibody
    • Subtilisin like protease antibody
    • Subtilisin-like proprotein convertase 4 antibody
    • subtilisin/kexin like protease PACE4 antibody
    • Subtilisin/kexin-like protease PACE4 antibody
    see all

图片

  • ab110144, at 3.75 µg/ml, staining PACE4 in formalin fixed, paraffin embedded Human skeletal muscle by Immunohistochemistry.
  • ab110144, at 3.75 µg/ml, staining PACE4 in formalin fixed, paraffin embedded Human testis by Immunohistochemistry.
  • ab110144 (1/200) staining PACE4 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

文献

ab110144 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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