概述

  • 产品名称Anti-PABPN1抗体[EP3000Y]
    参阅全部 PABPN1 一抗
  • 描述
    兔单克隆抗体[EP3000Y] to PABPN1
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PABPN1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: Raw264.7, MCF-7 and HeLa cell lysates. ICC/IF: MCF-7 cells. Flow Cyt: MCF-7 cells. IHC-P: Squamous cell cervical carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-PABPN1 antibody (Alexa Fluor® 488) [EP3000Y] (ab206056)

    Anti-PABPN1 antibody (Alexa Fluor® 647) [EP3000Y] (ab206057)

性能

应用

Our Abpromise guarantee covers the use of ab75855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in PABPN1 are the cause of oculopharyngeal muscular dystrophy (OPMD) [MIM:164300]. OPMD is a form of late-onset slowly progressive myopathy characterized by eyelid ptosis, dysphagia and, sometimes by other cranial and limb-muscle involvement.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 结构域The RRM domain is essential for specific adenine bases recognition in the poly(A) tail but not sufficient for poly(A) binding.
  • 翻译后修饰Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties.
  • 细胞定位Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism. Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Nucleus; nuclear speckle. Colocalizes with SKIP and poly(A) RNA in nuclear speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Nuclear poly(A)-binding protein 1 antibody
    • OPMD antibody
    • PAB2 antibody
    • PABII antibody
    • PABP 2 antibody
    • pABP-2 antibody
    • PABP2 antibody
    • PABP2_HUMAN antibody
    • PABPII antibody
    • Pabpn1 antibody
    • poly(A) binding protein nuclear 1 antibody
    • Poly(A)-binding protein 2 antibody
    • Poly(A)-binding protein II antibody
    • PolyA binding protein II antibody
    • Polyadenylate-binding nuclear protein 1 antibody
    • Polyadenylate-binding protein 2 antibody
    see all

Anti-PABPN1 antibody [EP3000Y] 图像

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling PABPN1 with ab75855 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Lanes 1 - 2 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/200000 dilution
    Lane 3 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/1000000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size : 33 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing MCF-7 cells stained with ab75855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75855, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab75855, at 1/100 dilution, staining PABPN1 in squamous cell cervical carcinoma, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Anti-PABPN1 antibody [EP3000Y] (ab75855)参考文献

This product has been referenced in:
  • Bergeron D  et al. Regulated Intron Retention and Nuclear Pre-mRNA Decay Contribute to PABPN1 Autoregulation. Mol Cell Biol 35:2503-17 (2015). Read more (PubMed: 25963658) »
  • Davidson L  et al. 3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells. Genes Dev 28:342-56 (2014). Human . Read more (PubMed: 24478330) »

See all 8 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control Anti-hnRNP C antibody (sc-15386) bound protein G dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein G dynabeads without antibody bound
Immuno-precipitation step Other - Protein G dynabeads
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提交于 Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 30000 cells
Specification 293T
Gel Running Conditions Reduced Denaturing (4-20% Tris-Glycine)
Blocking step 5% Milk, 1% BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Feb 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"