Anti-PABPC3抗体(ab68090)

概述

  • 产品名称Anti-PABPC3抗体
    参阅全部 PABPC3 一抗
  • 描述
    小鼠多克隆抗体to PABPC3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    PABPC3 (1 a.a. ~ 631 a.a) full-length human protein.

  • 阳性对照
    • PABPC3 transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab68090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).

靶标

  • 功能Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1.
  • 组织特异性Testis specific.
  • 序列相似性Belongs to the polyadenylate-binding protein type-1 family.
    Contains 1 PABC domain.
    Contains 4 RRM (RNA recognition motif) domains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PABP-3 antibody
    • PABP3 antibody
    • PABP3_HUMAN antibody
    • PABPC3 antibody
    • PABPL3 antibody
    • Poly(A) binding protein 3 antibody
    • Poly(A)-binding protein 3 antibody
    • Polyadenylate binding protein 3 antibody
    • Polyadenylate-binding protein 3 antibody
    • RP11-165I9.5 antibody
    • Testis specific poly(A) binding protein antibody
    • Testis-specific poly(A)-binding protein antibody
    • tPABP antibody
    see all

Anti-PABPC3 antibody 图像

  • All lanes : Anti-PABPC3 antibody (ab68090) at 1/500 dilution

    Lane 1 : PABPC3 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 70 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

Anti-PABPC3 antibody (ab68090)参考文献

ab68090 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3µg/mL · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

提交于 Jan 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"