概述

  • 产品名称Anti-PABP2抗体[EP3001Y]
    参阅全部 PABP2 一抗
  • 描述
    兔单克隆抗体[EP3001Y] to PABP2
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide from Human PABP2

  • 阳性对照
    • WB: T47D, SKBR3, Ramos and Raji cell lysates; IHC: urinary bladder transitional cell carcinoma.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab81224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/60. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 相关性PABP2 is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Two different isoform have been identified.
    • 细胞定位Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • Nuclear poly(A) binding protein 1 antibody
      • PAB2 antibody
      • PABII antibody
      • PABP2 antibody
      • PABPN1 antibody
      • Poly(A) binding protein 2 antibody
      • Polyadenylate binding nuclear protein 1 antibody
      • Polyadenylate binding protein 2 antibody
      • ROX2 antibody
      see all

    Anti-PABP2 antibody [EP3001Y] 图像

    • All lanes : Anti-PABP2 antibody [EP3001Y] (ab81224) at 1/20000 dilution

      Lane 1 : T47D cell lysates
      Lane 2 : SKBR3 cell lysates
      Lane 3 : Ramos cell lysates
      Lane 4 : Raji cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 33 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry staining of paraffin-embedded urinary bladder transitional cell carcinoma tissue, using 1/100 ab81224
    • Overlay histogram showing HeLa cells stained with ab81224 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81224, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Anti-PABP2 antibody [EP3001Y] (ab81224)参考文献

    This product has been referenced in:
    • Kim SH  et al. ALS-associated proteins TDP-43 and FUS/TLS function in a common biochemical complex to coregulate HDAC6 mRNA. J Biol Chem : (2010). WB ; Human . Read more (PubMed: 20720006) »
    • Kim SH  et al. Amyotrophic lateral sclerosis-associated proteins TDP-43 and FUS/TLS function in a common biochemical complex to co-regulate HDAC6 mRNA. J Biol Chem 285:34097-105 (2010). Read more (PubMed: 20720006) »

    See all 2 Publications for this product

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 2e+006 cells
    Gel Running Conditions Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
    Sample Cow Cell lysate - whole cell (bovine PABPN1 expressed in yeast S. cerevisiae cel)
    Specification bovine PABPN1 expressed in yeast S. cerevisiae cel
    Blocking step (agent) for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Jun 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"