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Our Abpromise guarantee covers the use of ab6125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17977970|
|Flow Cyt||Use 1µg for 106 cells.|
This image is courtesy of an abreview submitted by Francisco Ramirez-ValleThe cells were lysed with NP40 buffer with protease inhibitor cocktail, 10 micrograms of protein were separated by SDS-PAGE and transferred to PVDF membrane, blocked and blotted for two hours with PABP antibody in TBST, washed three times, secondary antibody for 1 hr (goat anti-mouse, Amersham, 1:10000).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"