概述

  • 产品名称Anti-p95 NBS1抗体[NBS1-501]
    参阅全部 p95 NBS1 一抗
  • 描述
    小鼠单克隆抗体[NBS1-501] to p95 NBS1
  • 经测试应用适用于: ICC, WB, IP, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide:

    GSKNVDLSGRQERKQ

    , corresponding to amino acids 206-220 of Human p95 NBS1

  • 阳性对照
    • Nuclear extract of HEK 293T expressing recombinant human NBS1

性能

应用

Our Abpromise guarantee covers the use of ab49958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
WB
IP
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 2 - 4 µg/ml. Predicted molecular weight: 85 kDa.
    Array: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
    • 组织特异性Ubiquitous. Expressed at high levels in testis.
    • 疾病相关Nijmegen breakage syndrome
      Breast cancer
      Aplastic anemia
      Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
    • 序列相似性Contains 1 BRCT domain.
      Contains 1 FHA domain.
    • 结构域The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
      The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
      The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
    • 翻译后修饰Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
    • 细胞定位Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AT V1 antibody
      • AT V2 antibody
      • ATV antibody
      • Cell cycle regulatory protein p95 antibody
      • FLJ10155 antibody
      • MGC87362 antibody
      • Nbn antibody
      • NBN_HUMAN antibody
      • NBS 1 antibody
      • NBS antibody
      • NBS1 antibody
      • Nibrin antibody
      • Nijmegen breakage syndrome 1 (nibrin) antibody
      • Nijmegen breakage syndrome antibody
      • Nijmegen breakage syndrome protein 1 antibody
      • p95 antibody
      • p95 protein of the MRE11/RAD50 complex antibody
      see all

    Anti-p95 NBS1 antibody [NBS1-501] (ab49958)参考文献

    This product has been referenced in:
    • Nie C  et al. Multifunctional assembly of micrometer-sized colloids for cell sorting. Small 11:2555-63 (2015). Read more (PubMed: 25613156) »
    • Cilli D  et al. Identification of the interactors of human nibrin (NBN) and of its 26 kDa and 70 kDa fragments arising from the NBN 657del5 founder mutation. PLoS One 9:e114651 (2014). WB . Read more (PubMed: 25485873) »

    See all 4 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab49958.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"