Anti-p90 Autoantigen抗体(ab99518)


  • 产品名称Anti-p90 Autoantigen抗体
    参阅全部 p90 Autoantigen 一抗
  • 描述
    兔多克隆抗体to p90 Autoantigen
  • 经测试应用适用于: WB, IP, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 853-903 of Human p90 Autoantigen (NP_065941.1).

  • 阳性对照
    • HeLa and 293T cell lines This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDAMB231


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab99518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


Anti-p90 Autoantigen antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling p90 Autoantigen with ab99518 at 1/200 (1µg/ml). Detection: DAB.
  • ab99518 stained MDAMB231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99518 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-p90 Autoantigen antibody (ab99518) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 102 kDa

    Exposure time : 3 seconds
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99518 at 3 µg/mg lysate; 20% of immunoprecipitate loaded

    Western Blot: p90 Autoantigen antibody (ab99518) at 0.1 µg/ml

    developed using the ECL technique

    Exposure time : 3 seconds

    Predicted band size : 102 kDa

Anti-p90 Autoantigen antibody (ab99518)参考文献

ab99518 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99518.
Please use the links above to contact us or submit feedback about this product.