Anti-p90 Autoantigen抗体(ab99518)


  • 产品名称
    Anti-p90 Autoantigen抗体
    参阅全部 p90 Autoantigen 一抗
  • 描述
    兔多克隆抗体to p90 Autoantigen
  • 经测试应用
    适用于: WB, IP, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 853-903 of Human p90 Autoantigen (NP_065941.1).

  • 阳性对照
    • HeLa and 293T cell lines This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDAMB231


  • 形式
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab99518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能
    Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
  • 组织特异性
    Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
  • 细胞定位
    Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancerous inhibitor of PP2A antibody
    • Cancerous Inhibitor of Protein Phosphatase 2A antibody
    • CIP2A antibody
    • CIP2A_HUMAN antibody
    • FLJ12850 antibody
    • Kiaa1524 antibody
    • MGC163436 antibody
    • p90 antibody
    • p90 autoantigen antibody
    • Protein CIP2A antibody
    see all

Anti-p90 Autoantigen antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling p90 Autoantigen with ab99518 at 1/200 (1µg/ml). Detection: DAB.
  • ab99518 stained MDAMB231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99518 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-p90 Autoantigen antibody (ab99518) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 102 kDa

    Exposure time : 3 seconds
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99518 at 3 µg/mg lysate; 20% of immunoprecipitate loaded

    Western Blot: p90 Autoantigen antibody (ab99518) at 0.1 µg/ml

    developed using the ECL technique

    Exposure time : 3 seconds

    Predicted band size : 102 kDa

Anti-p90 Autoantigen antibody (ab99518)参考文献

ab99518 has not yet been referenced specifically in any publications.

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