概述

  • 产品名称
    Anti-p90 Autoantigen抗体
    参阅全部 p90 Autoantigen 一抗
  • 描述
    兔多克隆抗体to p90 Autoantigen
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within the amino acids 550-600 of Human p90 Autoantigen, NP_065941.1

  • 阳性对照
    • Human colon carcinoma. Human ovarian carcinoma.
  • 常规说明

    Concentration is optimized for IHC and not determined

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
  • 组织特异性
    Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
  • 细胞定位
    Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancerous inhibitor of PP2A antibody
    • Cancerous Inhibitor of Protein Phosphatase 2A antibody
    • CIP2A antibody
    • CIP2A_HUMAN antibody
    • FLJ12850 antibody
    • Kiaa1524 antibody
    • MGC163436 antibody
    • p90 antibody
    • p90 autoantigen antibody
    • Protein CIP2A antibody
    see all

Anti-p90 Autoantigen antibody 图像

  • Detection of Human p90 Autoantigen by Immunohistochemistry.
    Sample: FFPE section of Human colon carcinoma.
    Antibody: ab84547 used at a dilution of 1/250.
    Detection: DAB staining.
  • Detection of Human p90 Autoantigen by Immunohistochemistry.
    Sample: FFPE section of Human ovarian carcinoma.
    Antibody: ab84547 used at a dilution of 1/250.
    Detection: DAB staining.
  • Immunohistochemical analysis of Human breast tumour tissue, staining p90 Autoantigen with ab84547 at 1/100 dilution.

Anti-p90 Autoantigen antibody (ab84547)参考文献

This product has been referenced in:
  • Liu CY  et al. Tamoxifen induces apoptosis through cancerous inhibitor of protein phosphatase 2A dependent phospho-Akt inactivation in estrogen-receptor negative human breast cancer cells. Breast Cancer Res 16:431 (2014). IHC-P . Read more (PubMed: 25228280) »
  • Yu HC  et al. Erlotinib derivative inhibits hepatocellular carcinoma by targeting CIP2A to reactivate protein phosphatase 2A. Cell Death Dis 5:e1359 (2014). IHC-P . Read more (PubMed: 25077545) »

See all 4 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: RT°C
Antigen retrieval step
None
Sample
Human Tissue sections (Breast)
Specification
Breast
Permeabilization
No
Fixative
Formaldehyde
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提交于 Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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