概述

  • 产品名称
    Anti-p8抗体
  • 描述
    兔多克隆抗体to p8
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    ERKLVTKLQNSERKKRGARR

    , corresponding to amino acids 62-82 of Human p8

性能

应用

Our Abpromise guarantee covers the use of ab6028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 8 kDa (predicted molecular weight: 8 kDa).

靶标

  • 功能
    Could participate in the response to proapoptotic stimuli and promotes cellular growth in a way that helps the tissue counteract diverse injuries. May contribute to the metastatic phenotype.
  • 组织特异性
    Highly expressed in pancreas.
  • 翻译后修饰
    Phosphorylated.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Candidate of metastasis 1 antibody
    • COM1 antibody
    • Nuclear protein 1 antibody
    • Nupr1 antibody
    • NUPR1_HUMAN antibody
    • p8 protein antibody
    • Protein p8 antibody
    see all

实验方案

文献

This product has been referenced in:
  • Palam LR  et al. Integrated stress response is critical for gemcitabine resistance in pancreatic ductal adenocarcinoma. Cell Death Dis 6:e1913 (2015). WB . Read more (PubMed: 26469962) »

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human colon cancer cell)
Loading amount
30 µg
Specification
human colon cancer cell
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 09 2013

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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Thank you for your enquiry. One difference that I see between these two antibodies is that ab6028 has more detail for the immunogen sequence "Synthetic peptide: ERKLVTKLQNSERKKRGARR, corresponding to amino acids 62-82 of Human p8" and ab6027 says that ...

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I'm sorry to hear you are having problem with ab6028. P8 is a nuclear protein, do you do nuclear extraction to have a concentrated sample to load onto your gels? I would like to suggest an overnight incubation of the primary antibody (trying 1:...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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