Anti-p75 NGF Receptor抗体(ab8874)

概述

  • 产品名称Anti-p75 NGF Receptor抗体
    参阅全部 p75 NGF Receptor 一抗
  • 描述
    兔多克隆抗体to p75 NGF Receptor
  • 特异性This antibody recognises mouse NGF Receptor
  • 经测试应用适用于: ICC/IF, Dot blot, IHC-P, IHC-Fr, IP, WB, IHC-FoFr, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    An extracellular fragment from the mouse p75 receptor (amino acids 43-161).

性能

应用

Our Abpromise guarantee covers the use of ab8874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/400. PubMed: 169737171/100.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-FoFr 1/100.
ICC 1/500.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • 序列相似性Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • 结构域Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • 翻译后修饰N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Anti-p75 NGF Receptor antibody 图像

  • ab11382 staining normal mouse brain tissue sections by IHC-FoFr.  Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C.  The primary antibody was diluted 1/100 and incubated with the sample for 18 hours at 4°C.  An Alexa Fluor® 555 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary.  This image (taken at 80x) demonstrates p75 positive neurons in the basal forebrain.

    See Abreview

  • Anti-p75 NGF Receptor antibody (ab8874) at 1/500 dilution + NIH-3T3 cells at 10 µg

    Secondary
    HRP-conjugated Donkey anti-Rabbit polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8874 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result). 

  • Anti-p75 NGF Receptor antibody (ab8874) at 1/500 dilution + whole cell lysate prepared from human WiDr colorectal cancer cell line at 5 µg

    Secondary
    HRP conjugated donkey anti-rabbit polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Exposure time : 12 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-p75 NGF Receptor antibody (ab8874)参考文献

This product has been referenced in:
  • Blanchard JW  et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci 18:25-35 (2015). IHC (PFA fixed) ; Mouse . Read more (PubMed: 25420069) »
  • Zhang J  et al. Effects of p75 neurotrophin receptor on regulating hypoxia-induced angiogenic factors in retinal pigment epithelial cells. Mol Cell Biochem 398:123-34 (2015). WB, IHC ; Human . Read more (PubMed: 25200140) »

See all 21 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 18 2014

ab8874 was raised against a synthetic peptide containing a GST tag. However, ab8875 is the immunogen affinity purified version of ab8874 and was purified to remove all antibody that recognizes the GST tag, so only antibody recognizing p75 NGF receptor ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (nerve)
Loading amount 25 µg
Specification nerve
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Mesenchymal stem cell)
Specification Mesenchymal stem cell
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Steven Johnstone

Verified customer

提交于 Sep 05 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your enquiry.

I can confirm that ab8874 Anti-p75 NGF Receptor antibody is sold as whole antiserum.

Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a co...

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Thank you for contacting us. I am sorry to hear that you are experiencing problems with this antibody.

The FACS protocol we have used for this antibody is very simple. Please find attached the document.

You may consider following s...

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TThank you for calling Abcam regarding positive controls.

AB10404 has been used in IHC-P on lung sections in the following publication:Liu Yet al.Human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated ...

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Thank you for providing that extra information. I now understanf more fully what the customer is trying to achieve and the problems they have been encountering.

As I mentioned in my previous email, the two lots used by the customer are from t...

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Thank you for your call today and for letting us know about the trouble with this new vial of ab8874.

As we discussed, I'm sending a free of charge vial on the order ***, which should arrive tomorrow. Please keep me updated about the results ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"