Anti-p73 (phospho Y99)抗体(ab38457)

概述

  • 产品名称Anti-p73 (phospho Y99)抗体
    参阅全部 p73 一抗
  • 描述
    兔多克隆抗体to p73 (phospho Y99)
  • 特异性ab38457 recognises endogenous levels of p73 only when phosphorylated at tyrosine 99.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human p73 around the phosphorylation site of tyrosine 99 (SPYpAQ).

  • 阳性对照
    • Human lung carcinoma tissue, extracts from K562 cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab38457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 70 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
  • 组织特异性Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
  • 序列相似性Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • 结构域Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
    The WW-binding motif mediates interaction with WWOX.
  • 翻译后修饰Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
    Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
    Ubiquitinated; leading to its degradation by the proteasome.
  • 细胞定位Nucleus. Accumulates in the nucleus in response to DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • p53 like transcription factor antibody
    • p53 related protein antibody
    • p53-like transcription factor antibody
    • p53-related protein antibody
    • p73 antibody
    • P73_HUMAN antibody
    • TP73 antibody
    • Tumor protein p73 antibody
    see all

Anti-p73 (phospho Y99) antibody 图像

  • All lanes : Anti-p73 (phospho Y99) antibody (ab38457) at 1/500 dilution

    Lane 1 : untreated extracts from K562 cells
    Lane 2 : Pervanadate treated extracts from K562 cells

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 70 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of p73 (phospho Y99) in paraffin embedded human lung carcinoma tissue sections using 1/50 ab38457. Left: untreated sample. Right: sample pretreated with phosphopeptide (negative control).

Anti-p73 (phospho Y99) antibody (ab38457)参考文献

ab38457 has not yet been referenced specifically in any publications.

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