Anti-p73 Delta N抗体[38C674] - ChIP Grade (ab13649)

概述

  • 产品名称Anti-p73 Delta N抗体[38C674] - ChIP Grade
  • 描述
    小鼠单克隆抗体[38C674] to p73 Delta N - ChIP Grade
  • 特异性This antibody does not cross react with full-length p73.
  • 经测试应用适用于: ChIP, Flow Cyt, IHC-P, IHC-Fr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    LYVGDPARHLAT

    , corresponding to amino acids 2-13 of Human p73 Delta N.

  • 表位N-terminal domain.

性能

应用

Our Abpromise guarantee covers the use of ab13649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use 1µg for 106 cells.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性Even though the tumor suppressor gene p53 is highly important in human cancer, as indicated by the fact that it is mutated in about 50% of cases, up to a few years ago no similar proteins had been identified. Two p53 homologues are now identified, p73 and p63, with high amino acid identity suggesting similar functions. Both p63 and p73 have two significant structural peculiarities compared with p53; the presence of an extended non-conserved C-terminus containing a sterile alpha motive (SAM), (typical of developmental proteins), and the presence of number of different splicing isoforms differing in the N-terminus or in the absence of the transactivation domain (delta N forms).
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Delta N p73 antibody
    • DNp73 antibody
    • p53 like transcription factor antibody
    • p53 related protein antibody
    • P73 antibody
    • TP73 antibody
    see all

Anti-p73 Delta N antibody [38C674] - ChIP Grade 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung squamous cell carcinoma tissue labelling p73 Delta N with ab13649 at 5µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

  • All lanes : Anti-p73 Delta N antibody [38C674] - ChIP Grade (ab13649) at 1 µg/ml

    Lane 1 : The lysate of a cell line transfected with dNp73 cDNA
    Lane 2 : HeLa cell lysate
    Lane 3 : NIH 3T3 cell lysate


    Predicted band size : 73 kDa
    Western blot analysis for dNp73 using ab13649 at 1 ug/ml in A) a cell line transfected with dNp73 cDNA, B) HeLa, and C) NIH 3T3 cell lysate.
  • IHC analysis of p73 Delta N in embryonic day 15 (A) and day 17 (B) E15 rat embryonic kidney at 1:2000. p73 Delta N is expressed in ureteric bud (UB) branches, and metanephrogenic mesenchyme (MM), as well as in cells invading the glomerular cleft. p73 Delta N is expressed in the ureteric bud (UB) branches, metanephrogenic mesenchyme (MM), and nephron progenitors.
  • Overlay histogram showing HeLa cells stained with ab13649 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13649, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-p73 Delta N antibody [38C674] - ChIP Grade (ab13649)参考文献

This product has been referenced in:
  • Hanks TS & Gauss KA Pleomorphic adenoma gene-like 2 regulates expression of the p53 family member, p73, and induces cell cycle block and apoptosis in human promonocytic U937 cells. Apoptosis 17:236-47 (2012). WB ; Human . Read more (PubMed: 22076304) »
  • Wu H  et al. Pirh2, a ubiquitin E3 ligase, inhibits p73 transcriptional activity by promoting its ubiquitination. Mol Cancer Res 9:1780-90 (2011). Read more (PubMed: 21994467) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Whole Embryo)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Treatment 400 mg/kg and 600 mg/kg Hydroxyurea for 3 hours
Specification Whole Embryo
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jan 12 2016

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Tous les produits de notre catalogue qui sont marqués comme "ChIP grade" ont été testés en immunoprécipitation de chromatine (ChIP). Nous garantissons donc tou...

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