概述

  • 产品名称Anti-p73 alpha+beta抗体[ER-15]
  • 描述
    小鼠单克隆抗体[ER-15] to p73 alpha+beta
  • 经测试应用适用于: IHC-P, IP, ICC/IF, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Recombinant fragment, corresponding to amino acids 380-637 of Monkey p73 alpha+beta.

  • 阳性对照
    • COS monkey kidney cells and IM9 human myeloma cells
  • 常规说明


    This antibody does not cross-react with p53 or p53-related p51/Ket protein. This antibody binds to both p73a and p73b in immunoprecipitation and Western Blot assays.

性能

应用

Our Abpromise guarantee covers the use of ab17230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 17079275
IP Use a concentration of 2 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 70-80 kDa.
EMSA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性The p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. This antibody does not cross-react with p53 or p53-related p51/Ket protein. p73 is expressed in either a full-length form MW ~80kDa or a shorter MW ~70kDa mRNA variant, with exon 13 spliced out. This antibody binds to both p73a and p73b in immunoprecipitation and Western Blot assays. Transient overexpression of p73 in cells can induce apoptosis and p21, a cellular p53 target gene primarily responsible for p53-dependent cell cycle arrest.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • p53 like transcription factor antibody
    • p53 related protein antibody
    • P73 antibody
    • TP73 antibody
    • Tumor protein p73 antibody
    see all

Anti-p73 alpha+beta antibody [ER-15] 图像

  • Overlay histogram showing HEK293 cells stained with ab17230 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17230, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-p73 alpha+beta antibody [ER-15] (ab17230)参考文献

This product has been referenced in:
  • King KE  et al. Dysregulated ?Np63a negatively regulates the maspin promoter in keratinocytes via blocking endogenous p73 binding. Mol Carcinog 53:698-710 (2014). Mouse . Read more (PubMed: 23475637) »
  • Wu H  et al. Pirh2, a ubiquitin E3 ligase, inhibits p73 transcriptional activity by promoting its ubiquitination. Mol Cancer Res 9:1780-90 (2011). Read more (PubMed: 21994467) »

See all 5 Publications for this product

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