Anti-P70 S6 Kinase beta抗体(ab70271)

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概述

  • 产品名称
    Anti-P70 S6 Kinase beta抗体
    参阅全部 P70 S6 Kinase beta 一抗
  • 描述
    兔多克隆抗体to P70 S6 Kinase beta
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between C terminal amino acids 430-482 of Human P70 S6 Kinase beta (NP_003943.2)

  • 阳性对照
    • HeLa whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-6 µg/mg of lysate.

靶标

  • 功能
    Phosphorylates specifically ribosomal protein S6.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • 70 kDa ribosomal protein S6 kinase 2 antibody
    • EC 2.7.11.1 antibody
    • KS6B2_HUMAN antibody
    • p70 beta antibody
    • p70 ribosomal S6 kinase beta antibody
    • p70 S6 kinase beta antibody
    • p70 S6K-beta antibody
    • p70 S6KB antibody
    • p70 S6Kbeta antibody
    • p70(S6K) beta antibody
    • p70-beta antibody
    • p70-S6K 2 antibody
    • P70S6K2 antibody
    • p70S6Kb antibody
    • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
    • Ribosomal protein S6 kinase B2 antibody
    • Ribosomal protein S6 kinase beta 2 antibody
    • Ribosomal protein S6 kinase beta-2 antibody
    • Rps6kb2 antibody
    • S6 kinase related kinase antibody
    • S6 kinase-related kinase antibody
    • S6K beta 2 antibody
    • S6K beta antibody
    • S6K-beta antibody
    • S6K-beta-2 antibody
    • S6K2 antibody
    • Serine/threonine protein kinase 14 beta antibody
    • Serine/threonine-protein kinase 14B antibody
    • SRK antibody
    • STK14B antibody
    see all

图片

  • Western blot - Anti-P70 S6 Kinase beta antibody (ab70271)
    Western blot - Anti-P70 S6 Kinase beta antibody (ab70271)
    All lanes : Anti-P70 S6 Kinase beta antibody (ab70271) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 15 µg
    Lane 2 : HeLa whole cell lysate at 5 µg

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 35 kDa, 67 kDa, 69 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

文献

ab70271 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab70271？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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1 Review or Q&A

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Hi Heather,

Thank you for getting back to me!

Yes, we did see other size bands. I’ve scanned in the blot and attached it for you to see.

I loaded 100ug of protein (I know...Lots!).

We have a lysis buf...

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Thank you for the additional protocol details and for the image provided. Have you tried any shorter incubations with the primary, such as 1 hr? Although we normally recommend ON for the strongest signal, reducing this time may eliminate some of the ba...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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