Anti-P70 S6 Kinase alpha (phospho T229)抗体(ab59208)

概述

  • 产品名称Anti-P70 S6 Kinase alpha (phospho T229)抗体
    参阅全部 P70 S6 Kinase alpha 一抗
  • 描述
    兔多克隆抗体to P70 S6 Kinase alpha (phospho T229)
  • 特异性Detects endogenous levels of P70 S6 Kinase alpha only when phosphorylated at threonine 229 for human and rat, threonine 252 for mouse. This antibody was specifically designed for detection of S6K1 and binds to the protein kinase domain of isoforms alpha I and alpha II. We have some feedback to indicate that is may not be able to detect S6K2, but this has not been confirmed.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 229 (THTPFC)

  • 阳性对照
    • Human brain. Jurkat cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab59208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 相关性P70 S6 Kinase alpha is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and contains 2 non-identical kinase catalytic domains. It phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein is activated by serine/threonine phosphorylation and protein kinase C and inactivated by type 2A phosphatase. Activation leads to an increase in protein synthesis and cell proliferation. Overexpression of this kinase is involved in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
  • 细胞定位Cytoplasmic and synaptosome
  • 数据库链接
  • 别名
    • 70 kDa ribosomal protein S6 kinase 1 antibody
    • EC 2.7.11.1 antibody
    • KS6B1 antibody
    • p70 alpha antibody
    • p70 antibody
    • p70 S6 kinase alpha antibody
    • p70 S6K antibody
    • p70(S6K)-alpha antibody
    • p70S6K antibody
    • PS6K antibody
    • ribosomal protein S6 kinase beta-1 antibody
    • Ribosomal protein S6 kinase I antibody
    • Ribosomal protein S6, 70-KD, 1 antibody
    • RPS6KB1 antibody
    • S6K antibody
    • S6K-beta-1 antibody
    • S6K1 antibody
    • serine/threonine kinase 14 alpha antibody
    • serine/threonine-protein kinase 14A antibody
    • STK14A antibody
    see all

Anti-P70 S6 Kinase alpha (phospho T229) antibody 图像

  • All lanes : Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunizing phosphopeptide


    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
  • Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    This image is a courtesy of Anonymous Abreview

    See Abreview

Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208)参考文献

ab59208 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080 cell line)
Loading amount 10 µg
Specification HT1080 cell line
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Apr 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cell line)
Loading amount 10 µg
Specification NIH 3T3 cell line
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Nov 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"