概述
- 产品名称Anti-p66 beta抗体
参阅全部 p66 beta 一抗 - 描述兔多克隆抗体to p66 beta
- 经测试应用适用于: IHC-P, WB, IPmore details
- 种属反应性与反应: Mouse, Human
预测可用于: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
- 免疫原
Synthetic peptide corresponding to a region between residues 50 and 100 of human p66 beta (NP_065750.1)
- 阳性对照
- HeLa and 293T whole cell lysates.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明ab76925 was affinity purified using an epitope specific to p66 beta immobilized on solid support.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab76925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 66 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
靶标
- 功能Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
- 组织特异性Ubiquitous.
- 序列相似性Contains 1 GATA-type zinc finger.
- 结构域CR1 is required for interaction with MBD3 and transcription repression.
CR2 is required for localization to discrete loci in the nucleus. - 细胞定位Nucleus. Nuclear, in discrete foci.
- Information by UniProt
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数据库链接
- Entrez Gene: 57459 Human
- Entrez Gene: 229542 Mouse
- Entrez Gene: 310614 Rat
- SwissProt: Q8WXI9 Human
- SwissProt: Q8VHR5 Mouse
- Unigene: 4779 Human
- Unigene: 596854 Human
- Unigene: 270999 Mouse
see all -
别名
- FLJ37346 antibody
- GATA zinc finger domain containing 2B antibody
- GATA zinc finger domain containing protein 2B antibody
see all
Anti-p66 beta antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p66 beta antibody (ab76925)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling p66 beta with ab76925 at 1/200 (1µg/ml). Detection: DAB. -
Western blot - p66 beta antibody (ab76925)All lanes : Anti-p66 beta antibody (ab76925) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 66 kDa
Observed band size : 72 kDa (why is the actual band size different from the predicted?)
Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - p66 beta antibody (ab76925)ab76925, at 1µg/ml, staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab76925 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Anti-p66 beta antibody (ab76925)参考文献
ab76925 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"