Anti-GATAD2B抗体(ab76924)
Key features and details
- Rabbit polyclonal to GATAD2B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GATAD2B抗体
参阅全部 GATAD2B 一抗 -
描述
兔多克隆抗体to GATAD2B -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant -
免疫原
Synthetic peptide corresponding to Human GATAD2B aa 1-50 (N terminal).
Database link: NP_065750.1 -
阳性对照
- WB: HeLa, 293T and CT26-WT whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76924于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus. -
组织特异性
Ubiquitous. -
序列相似性
Contains 1 GATA-type zinc finger. -
结构域
CR1 is required for interaction with MBD3 and transcription repression.
CR2 is required for localization to discrete loci in the nucleus. -
细胞定位
Nucleus. Nuclear, in discrete foci. - Information by UniProt
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数据库链接
- Entrez Gene: 57459 Human
- Entrez Gene: 229542 Mouse
- Entrez Gene: 310614 Rat
- Omim: 614998 Human
- SwissProt: Q8WXI9 Human
- SwissProt: Q8VHR5 Mouse
- Unigene: 4779 Human
- Unigene: 596854 Human
see all -
别名
- FLJ37346 antibody
- GATA zinc finger domain containing 2B antibody
- GATA zinc finger domain containing protein 2B antibody
see all
图片
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All lanes : Anti-GATAD2B antibody (ab76924) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 66 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
All lanes : Anti-GATAD2B antibody (ab76924) at 1/2000 dilution
Lane 1 : NIH 3T3 (Mouse embryonic fibroblasts) whole cell lysate
Lane 2 : TCMK-1 (Transformed C3H Mouse Kidney-1) whole cell lysate
Lane 3 : CT26-WT (Mouse colon carcinoma cell - Wild Type) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of GATAD2B by Western Blot of Immunprecipitate.
ab76924 at 1µg/ml staining GATAD2B in HeLa whole cell lysate immunoprecipitated using ab76924 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab76924 被引用在 2 文献中.
- Chalei V et al. The long non-coding RNA Dali is an epigenetic regulator of neural differentiation. Elife 3:e04530 (2014). PubMed: 25415054
- Cui S et al. Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic {beta}-Type Globin Promoters in Differentiated Adult Erythroid Cells. Mol Cell Biol 31:3298-311 (2011). PubMed: 21670149