兔多克隆抗体to p66 beta
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide corresponding to a region between residues 543 and 593 of human p66 beta (NP_065750.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab75280 was affinity purified using an epitope specific to p66beta immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
Contains 1 GATA-type zinc finger.
CR1 is required for interaction with MBD3 and transcription repression.
CR2 is required for localization to discrete loci in the nucleus.
Nucleus. Nuclear, in discrete foci.
Information by UniProt
GATA zinc finger domain containing 2B antibody
GATA zinc finger domain containing protein 2B antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p66 beta antibody (ab75280)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling p66 beta with ab75280 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation - p66 beta antibody (ab75280)
Detection of p66 beta by Western Blot of Immunprecipitate.
anti-p66 beta at 1µg/ml staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab75280 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"