概述

  • 产品名称Anti-p66 beta抗体
    参阅全部 p66 beta 一抗
  • 描述
    兔多克隆抗体to p66 beta
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 543 and 593 of human p66 beta (NP_065750.1)

  • 阳性对照
    • HeLa whole cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab75280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
    • 组织特异性Ubiquitous.
    • 序列相似性Contains 1 GATA-type zinc finger.
    • 结构域CR1 is required for interaction with MBD3 and transcription repression.
      CR2 is required for localization to discrete loci in the nucleus.
    • 细胞定位Nucleus. Nuclear, in discrete foci.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ37346 antibody
      • GATA zinc finger domain containing 2B antibody
      • GATA zinc finger domain containing protein 2B antibody
      • GATA zinc finger domain-containing protein 2B antibody
      • GATAD2B antibody
      • KIAA1150 antibody
      • MGC138257 antibody
      • MGC138285 antibody
      • mKIAA1150 antibody
      • p66/p68 antibody
      • P66B_HUMAN antibody
      • P66beta antibody
      • RGD1308533 antibody
      • transcription repressor p66 beta component of the MeCP1 complex antibody
      • Transcriptional repressor p66 beta antibody
      • Transcriptional repressor p66-beta antibody
      see all

    Anti-p66 beta antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling p66 beta with ab75280 at 1/200 (1µg/ml). Detection: DAB.
    • Detection of p66 beta by Western Blot of Immunprecipitate. anti-p66 beta at 1µg/ml staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab75280 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

    Anti-p66 beta antibody (ab75280)参考文献

    ab75280 has not yet been referenced specifically in any publications.

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