产品名称 Anti-p66 beta抗体 参阅全部 p66 beta 一抗
兔多克隆抗体to p66 beta
IHC-P, IP more details 不适用于:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide corresponding to a region between residues 543 and 593 of human p66 beta (NP_065750.1)
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab75280 was affinity purified using an epitope specific to p66beta immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
应用说明 Is unsuitable for WB.
功能 Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
序列相似性 Contains 1 GATA-type zinc finger.
结构域 CR1 is required for interaction with MBD3 and transcription repression. CR2 is required for localization to discrete loci in the nucleus.
细胞定位 Nucleus. Nuclear, in discrete foci.
Information by UniProt
GATA zinc finger domain containing 2B antibody
GATA zinc finger domain containing protein 2B antibody
Anti-p66 beta antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p66 beta antibody (ab75280)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling p66 beta with ab75280 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation - p66 beta antibody (ab75280)
Detection of p66 beta by Western Blot of Immunprecipitate.
anti-p66 beta at 1µg/ml staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab75280 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Anti-p66 beta antibody (ab75280)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"