Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling p66 alpha with ab87663 at 1/1000 (1µg/ml). Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-p66 alpha antibody (ab87663)This image is courtesy of an anonymous Abreview
ab87663 staining p66 alpha in mouse MEL cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200 in TBS) for 12 hours at 4°C. An FITC-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Detection of p66 alpha by Western Blot of Immunprecipitate.
ab87663 at 1µg/ml staining p66 alpha in HeLa whole cell lysate immunoprecipitated using ab87663 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.