概述

  • 产品名称Anti-p63抗体[Y289]
    参阅全部 p63 一抗
  • 描述
    兔单克隆抗体[Y289] to p63
  • 特异性This antibody should detect both isoforms 2,9,10,11 and 12 of p63.
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p63 aa 650 to the C-terminus (C terminal).

  • 表位ab32353 reacts with an epitope located in the C terminal region of p63.
  • 阳性对照
    • WB: A431 cell lysate. IHC-P: Human skin tissue. ICC/IF: A431 cells.
  • 常规说明

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab32353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Predicted molecular weight: 77 kDa.

For unpurified use at 1/4000.

ICC/IF 1/100.

For unpurified use at 1/250 - 1/500.

IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.
    • 组织特异性Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
    • 疾病相关Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting.
      Defects in TP63 are the cause of ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]. AEC is an autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiform adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate.
      Defects in TP63 are the cause of ectrodactyly-ectodermal dysplasia-cleft lip/palate syndrome type 3 (EEC3) [MIM:604292]. EEC3 is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting.
      Defects in TP63 are the cause of split-hand/foot malformation type 4 (SHFM4) [MIM:605289]. Split-hand/split-foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. There is restricted overlap between the mutational spectra of EEC3 and SHFM4.
      Defects in TP63 are the cause of limb-mammary syndrome (LMS) [MIM:603543]. LMS is characterized by ectrodactyly, cleft palate and mammary-gland abnormalities.
      Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and ovarian cancers.
      Defects in TP63 are a cause of ectodermal dysplasia Rapp-Hodgkin type (EDRH) [MIM:129400]; also called Rapp-Hodgkin syndrome or anhidrotic ectodermal dysplasia with cleft lip/palate. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDRH is characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate.
      Defects in TP63 are the cause of non-syndromic orofacial cleft type 8 (OFC8) [MIM:129400]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
    • 序列相似性Belongs to the p53 family.
      Contains 1 SAM (sterile alpha motif) domain.
    • 结构域The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
    • 翻译后修饰May be sumoylated.
      Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AIS antibody
      • Amplified in squamous cell carcinoma antibody
      • B(p51A) antibody
      • B(p51B) antibody
      • Chronic ulcerative stomatitis protein antibody
      • CUSP antibody
      • DN p63 alpha 1 antibody
      • DNp63 antibody
      • EEC3 antibody
      • id:ibd3516 antibody
      • Keratinocyte transcription factor antibody
      • Keratinocyte transcription factor KET antibody
      • KET antibody
      • LMS antibody
      • MGC115972 antibody
      • MGC192897 antibody
      • NBP antibody
      • OFC8 antibody
      • OTTHUMP00000209732 antibody
      • OTTHUMP00000209733 antibody
      • OTTHUMP00000209734 antibody
      • OTTHUMP00000209735 antibody
      • OTTHUMP00000209737 antibody
      • OTTHUMP00000209738 antibody
      • OTTHUMP00000209739 antibody
      • OTTHUMP00000209740 antibody
      • OTTHUMP00000209741 antibody
      • OTTHUMP00000209742 antibody
      • OTTHUMP00000209743 antibody
      • OTTHUMP00000209744 antibody
      • p40 antibody
      • p51 antibody
      • P51/P63 antibody
      • p53-related protein p63 antibody
      • p53CP antibody
      • p63 antibody
      • P63_HUMAN antibody
      • p73H antibody
      • p73L antibody
      • RHS antibody
      • SHFM4 antibody
      • TAp63alpha antibody
      • TP53CP antibody
      • TP53L antibody
      • TP63 antibody
      • TP73L antibody
      • Transformation related protein 63 antibody
      • Transformation-related protein 63 antibody
      • Trp53rp1 antibody
      • Trp63 antibody
      • Tumor protein 63 antibody
      • Tumor protein p53-competing protein antibody
      • Tumor protein p53-like antibody
      • Tumor protein p63 antibody
      • Tumor protein p63 deltaN isoform delta antibody
      • Tumor protein p73 antibody
      • Tumor protein p73-like antibody
      see all

    Anti-p63 antibody [Y289] 图像

    • Anti-p63 antibody [Y289] (ab32353) at 1/4000 dilution (unpurified) + A431 cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 77 kDa
      Observed band size : 73 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-p63 antibody [Y289] (ab32353) at 1/10000 dilution (purified) + A431 cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 77 kDa
      Observed band size : 73 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular skin tissue labelling p63 with purified ab32353 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunocytochemsitry/Immunofluorescence analysis of A431 cells labelling p63 (red) with purified ab32353 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Immunofluorescent staining of A431 cells using ab32353 at 1/250 dilution.

    Anti-p63 antibody [Y289] (ab32353)参考文献

    This product has been referenced in:
    • Firth AL  et al. Generation of multiciliated cells in functional airway epithelia from human induced pluripotent stem cells. Proc Natl Acad Sci U S A 111:E1723-30 (2014). Read more (PubMed: 24706852) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Corneal limbal epithelial cells)
    Specification Corneal limbal epithelial cells
    Permeabilization Yes - Triton 0.3% - 5 minutes
    Fixative Methanol
    Username

    Mr. Manuel Chacon

    Verified customer

    提交于 Dec 18 2013

    Thank you for contacting us.

    Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

    For UNTESTED species and/or applications, we have established a testi...

    Read More

    Thank you for contacting us. I can provide the range in which the epitope is found. The peptide maps between
    amino acids 650 and 680 of human p63 (p73L) (UniProt: Q9H3D4), on the C-terminus. Please let me know if you have any questions.
    I hop...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"