Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Equus Cultured Cells (Equine Epidermal-derived stem cells)
Specification
Equine Epidermal-derived stem cells
Permeabilization
Yes - Triton X
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 21°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Goat-anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Also some cytoplasmatic staining in equine skin stem cells and their more differentiated progeny could be noticed using this marker.
Abcam response
We appreciate the data provided. Since this is the first time that the antibody has been tested on equine samples and the cytoplasmic staining is unexpected, we prefer to wait for more customer feedback before confirming cross reaction with this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Jan Spaas
Verified customer
提交于 Oct 08 2013