Anti-p60 CAF1抗体[EPR6105] (ab109442)


  • 产品名称Anti-p60 CAF1抗体[EPR6105]
    参阅全部 p60 CAF1 一抗
  • 描述
    兔单克隆抗体[EPR6105] to p60 CAF1
  • 经测试应用适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    A synthetic peptide corresponding to residues at the C-terminus of Human p60 CAF1.

  • 阳性对照
    • Jurkat, HeLa, 293T, and Caco2 cell lysates; Human colon and tonsil tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR6105
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab109442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • 功能Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
  • 序列相似性Belongs to the WD repeat HIR1 family.
    Contains 7 WD repeats.
  • 发展阶段Active complex is found in G1, S and G2 phases.
  • 翻译后修饰Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
  • 细胞定位Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAF 1 subunit B antibody
    • CAF 1A antibody
    • CAF I 60 kDa subunit antibody
    • CAF IP60 antibody
    • CAF-1 antibody
    • CAF-1 subunit B antibody
    • CAF-I 60 kDa subunit antibody
    • CAF-I p60 antibody
    • CAF1 antibody
    • CAF1A antibody
    • CAF1B_HUMAN antibody
    • CAF1P60 antibody
    • CHAF 1B antibody
    • CHAF1B antibody
    • Chromatin assembly factor 1 subunit B antibody
    • Chromatin assembly factor I antibody
    • Chromatin assembly factor I p60 subunit antibody
    • Human chromatin assembly factor-I p60 subunit antibody
    • M phase phosphoprotein 7 antibody
    • M-phase phosphoprotein 7 antibody
    • MPHOSPH7 antibody
    • MPP 7 antibody
    • MPP7 antibody
    • p60 subunit antibody
    see all

Anti-p60 CAF1 antibody [EPR6105] 图像

  • Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-p60 CAF1 antibody [EPR6105] (ab109442)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Caco2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 61 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab109442 at 1/250 dilution staining p60 CAF1 in paraffin-embedded Human colon tissue.
  • ab109442 at 1/250 dilution staining p60 CAF1 in paraffin-embedded Human tonsil tissue.

Anti-p60 CAF1 antibody [EPR6105] (ab109442)参考文献

ab109442 has not yet been referenced specifically in any publications.

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