概述

  • 产品名称Anti-p57 Kip2抗体[EP2515Y]
    参阅全部 p57 Kip2 一抗
  • 描述
    兔单克隆抗体[EP2515Y] to p57 Kip2
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p57 Kip2 aa 50-150 (N terminal).

  • 阳性对照
    • WB: HeLa, Jurkat and human, mouse and rat brain lysates. IHC-P: human breast carcinoma and placenta tissues. ICC/IF: HeLa cells. IP: HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-p57 Kip2 antibody (Alexa Fluor® 488) [EP2515Y] (ab199069)

    Anti-p57 Kip2 antibody (Alexa Fluor® 647) [EP2515Y] (ab199253)

    Anti-p57 Kip2 antibody (HRP) [EP2515Y] (ab199254)

性能

应用

Our Abpromise guarantee covers the use of ab75974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250.
WB 1/500. Predicted molecular weight: 32 kDa.
IP 1/30.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
    • 组织特异性Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
    • 疾病相关Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
      Note=Defects in CDKN1C are involved in tumor formation.
    • 序列相似性Belongs to the CDI family.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beckwith Wiedemann syndrome antibody
      • BWCR antibody
      • BWS antibody
      • CDKI antibody
      • CDKN 1C antibody
      • CDKN1C antibody
      • CDN1C_HUMAN antibody
      • Cyclin dependent kinase inhibitor 1C antibody
      • Cyclin dependent kinase inhibitor p57 antibody
      • Cyclin-dependent kinase inhibitor 1C antibody
      • Cyclin-dependent kinase inhibitor p57 antibody
      • KIP 2 antibody
      • KIP2 antibody
      • p57 antibody
      • p57 Kip 2 antibody
      • p57KIP2 antibody
      • WBS antibody
      see all

    Anti-p57 Kip2 antibody [EP2515Y] 图像

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling p57 Kip2 with purified ab75974 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

      Control: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    • Anti-p57 Kip2 antibody [EP2515Y] (ab75974) at 1/500 dilution (purified) + Human brain at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 57 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-p57 Kip2 antibody [EP2515Y] (ab75974) at 1/500 dilution (purified)

      Lane 1 : Mouse brain
      Lane 2 : Rat brain

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 32 kDa
      Observed band size : 57 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-p57 Kip2 antibody [EP2515Y] (ab75974) at 1/500 dilution (unpurified)

      Lane 1 : HeLa lysate
      Lane 2 : Jurkat lysate
      Lane 3 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 32 kDa
      Observed band size : 57 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling p57 Kip2 with purified ab75974 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • ab75974 staining p57 Kip2 in HeLa cells treated with dexamethasone (ab120743), by ICC/IF. Increase in p57 Kip2 expression correlates with increased concentration of dexamethasone as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120743 (dexamethasone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with unpurified ab75974 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    • ab75974 (purified) at 1/30 immunoprecipitating p57 Kip2 in HeLa cells treated with dexamethasone (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    Anti-p57 Kip2 antibody [EP2515Y] (ab75974)参考文献

    This product has been referenced in:
    • Li H  et al. Frs2a and Shp2 signal independently of Gab to mediate FGF signaling in lens development. J Cell Sci 127:571-82 (2014). Read more (PubMed: 24284065) »
    • Sun MM & Beier F Liver X Receptor activation delays chondrocyte hypertrophy during endochondral bone growth. Osteoarthritis Cartilage 22:996-1006 (2014). Mouse . Read more (PubMed: 24852699) »

    See all 7 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10%gel)
    Sample Mouse Tissue lysate - whole (hippocampus)
    Specification hippocampus
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Mar 30 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"