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Our Abpromise guarantee covers the use of ab53616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
Chromatin was obtained from Kc167 cells fixed with formaldehyde for 10 min. The ChIP was performed with 30-40 µg of chromatin, ab53616 at 1/300 dilution (incubated with samples for 16h at 4°C) and 20µl of Protein G Agarose beads per ChIP. The immunoprecipitated DNA was quantified by real time PCR (SYBR) . Gene A's enhancer region locus (positive control) shows enrichment when compared with it's coding sequence locus (negative control).
This image is courtesy of an Abreview submitted by Dr Kat Millen
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