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Synthetic peptide corresponding to Human p53 (intracellular). Synthetic phospho-peptide surrounding amino acid Ser15 of human p53
Database link: P04637
Our Abpromise guarantee covers the use of ab1431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. PubMed: 23365256|
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 53 kDa.|
|IP||Use a concentration of 5 - 10 µg/ml.|
ab1431 staining p53 in Mouse bone marrow WBCs cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab1431 staining p53 in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1:100 for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (green) ab150077) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).These pictures were taken in the different fields of the same bone marrow cell slide.